Lu Qi, Aihong Zhang, Yu Wang, Long Liu, and Xinghe Wang
Subjects
Paper, Enzyme-linked immunosorbent assay, Graphene oxide, Gold nanoparticles, Atom transfer radical polymer, Chemistry, QD1-999
Abstract
Abstract A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction.
Xinghe Wang, Lu Qi, Long Liu, Yu Wang, and Aihong Zhang
Subjects
Polymer modified, Paper, biology, Atom-transfer radical-polymerization, Graphene, Oxide, Nanoparticle, General Chemistry, Combinatorial chemistry, Primary and secondary antibodies, Atom transfer radical polymer, law.invention, lcsh:Chemistry, chemistry.chemical_compound, chemistry, Enzyme-linked immunosorbent assay, lcsh:QD1-999, law, Colloidal gold, Atom, biology.protein, Gold nanoparticles, Research Article, Graphene oxide
Abstract
A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. Electronic supplementary material The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users.
*ATOM transfer reactions, *POLYMERIZATION, *GRAPHENE oxide, *GOLD nanoparticles, *IMMUNOGLOBULINS
Abstract
A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. [ABSTRACT FROM AUTHOR]