1. Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses
- Author
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Kağan Huysal, Mehtap Bulut, Yasemin U Budak, and Murat Polat
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,Cardiac troponin ,business.industry ,Becton dickinson ,Emergency department ,AutoAnalyzer ,Creatine kinase.MB ,Pathology and Forensic Medicine ,Rapid serum tubes ,Deming regression ,Clotting time ,medicine ,Cardiac troponin T ,Creatine kinase-MB ,business ,Nuclear medicine ,Research Article - Abstract
Background In our emergency department, we collect blood in Rapid Serum Tubes (RSTs; Becton Dickinson, Franklin Lakes, NJ), in which clotting times are reduced. We investigated the influence of RST use on cardiac troponin T (hs-cTnT) and creatine kinase-MB (CK-MB) test results, in comparison with the use of tubes featuring a separator gel containing a clotting activator (SSTs; Green-vac, Yongin, Korea). Methods Samples from 60 patients were divided into equal aliquots and placed into RSTs and SSTs; hs-cTnT and CK-MB concentrations were determined using an autoanalyzer (Elecsys 2010) running commercial assays (Roche Diagnostics, Penzberg, Germany). Between-tube differences in CK-MB and hs-cTnT values were compared using the paired t-test, and correlations among variables were evaluated by calculation of Spearman correlation coefficients (r values). Deming regression analysis was performed and Bland-Altman plots were constructed. Results The hs-cTnT and CK-MB test results obtained from samples placed into RSTs and SSTs did not differ (p > 0.1). The correlations between the concentrations of hs-cTnT and CK-MB in samples placed into RSTs and SSTs were good; both r values were unity (p
- Published
- 2013
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