1. American ginseng (Panax quinquefolius) administration does not affect performance of the Roche COBAS Ampliprep/Taqman HIV-1 RNA assay
- Author
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Chun-Su Yuan, Adriana Andrade, Brent A. Bauer, Rahul P. Bakshi, Jeff A. Sloan, Todd T. Brown, and Antoine Simmons
- Subjects
Panax ,HIV Infections ,HIV 1 RNA assay ,complex mixtures ,Ginseng ,chemistry.chemical_compound ,TaqMan ,Medicine ,Humans ,American ginseng ,biology ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,RNA ,food and beverages ,General Medicine ,Reverse Transcription ,biology.organism_classification ,Virology ,Reverse transcriptase ,In vitro ,HIV Reverse Transcriptase ,Complementary and alternative medicine ,chemistry ,Ginsenoside ,HIV-1 ,RNA, Viral ,Reagent Kits, Diagnostic ,business ,Drugs, Chinese Herbal ,Research Article - Abstract
Background Previous data indicate that purified components of ginseng can inhibit HIV reverse transcriptase in vitro, suggesting that ginseng components in plasma may interfere with HIV-1 RNA detection assays. Methods Pre- and post-dose plasma from three volunteers dosed with 3000 mg American ginseng was spiked with HIV and analyzed by the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. Results Presence of American ginseng had no significant effect on measured HIV-1 RNA concentration. Variation within pre- and post-dose plasma pair was insignificant and within assay performance limits. Conclusion Plasma from subjects dosed with 3000 mg American ginseng does not interfere with the Roche COBAS Ampliprep/Taqman v2.0 HIV-1 RNA assay. This implies that in vitro inhibition of HIV reverse transcriptase by American ginseng components is unlikely to be clinically relevant.
- Published
- 2014