6 results on '"He, Feng"'
Search Results
2. Liver transcriptome analysis reveals extensive transcriptional plasticity during acclimation to low salinity in <italic>Cynoglossus semilaevis</italic>.
- Author
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Si, Yufeng, Wen, Haishen, Li, Yun, He, Feng, Li, Jifang, Li, Siping, and He, Huiwen
- Subjects
SALINITY ,ABIOTIC stress ,CYNOGLOSSIDAE ,FISH genomes ,OSMOTIC pressure - Abstract
Background: Salinity is an important abiotic stress that influences the physiological and metabolic activity, reproduction, growth and development of marine fish. It has been suggested that half-smooth tongue sole (
Cynoglossus semilaevis ), a euryhaline fish species, uses a large amount of energy to maintain osmotic pressure balance when exposed to fluctuations in salinity. To delineate the molecular response ofC. semilaevis to different levels of salinity, we performed RNA-seq analysis of the liver to identify the genes and molecular and biological processes involved in responding to salinity changes. Results: The present study yielded 330.4 million clean reads, of which 83.9% were successfully mapped to the reference genome ofC. semilaevis . One hundred twenty-eight differentially expressed genes (DEGs), including 43 up-regulated genes and 85 down-regulated genes, were identified. These DEGs were highly represented in metabolic pathways, steroid biosynthesis, terpenoid backbone biosynthesis, butanoate metabolism, glycerolipid metabolism and the 2-oxocarboxylic acid metabolism pathway. In addition, genes involved in metabolism, osmoregulation and ion transport, signal transduction, immune response and stress response, and cytoskeleton remodeling were affected during acclimation to low salinity. Genesacat2 ,fdps ,hmgcr ,hmgcs1 ,mvk ,pmvk ,ebp ,lss ,dhcr7 , anddhcr24 were up-regulated andabat ,ddc ,acy1 were down-regulated in metabolic pathways. Genesaqp10 andslc6a6 were down-regulated in osmoregulation and ion transport. Genesabat ,fdps ,hmgcs1 ,mvk ,pmvk and dhcr7 were first reported to be associated with salinity adaptation in teleosts. Conclusions: Our results revealed that metabolic pathways, especially lipid metabolism were important for salinity adaptation. The candidate genes identified from this study provide a basis for further studies to investigate the molecular mechanism of salinity adaptation and transcriptional plasticity in marine fish. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
3. Methylation levels at IGF2 and GNAS DMRs in infants born to preeclamptic pregnancies
- Author
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Danqing Yu, Yuan Jiang, Xiaoqun Ye, He-Feng Huang, Min Fang, Minyue Dong, Rong Fang, Jing He, Yun Liu, Hong Zhang, Aiping Zhang, Cong Han, and Jiamei Ge
- Subjects
Gestational hypertension ,Adult ,Male ,Risk ,medicine.medical_specialty ,Offspring ,Birth weight ,Mothers ,Preeclampsia ,GNAS ,Pre-Eclampsia ,Insulin-Like Growth Factor II ,Pregnancy ,Internal medicine ,medicine ,GNAS complex locus ,Genetics ,Chromogranins ,GTP-Binding Protein alpha Subunits, Gs ,Humans ,reproductive and urinary physiology ,Fetus ,DNA methylation ,biology ,Obstetrics ,IGF2 ,Gestational age ,Infant ,Metabolic diseases ,Hypertension, Pregnancy-Induced ,medicine.disease ,female genital diseases and pregnancy complications ,Endocrinology ,Cardiovascular Diseases ,embryonic structures ,biology.protein ,Female ,Biotechnology ,Research Article - Abstract
Background Offspring of pregnancy complicated with preeclampsia are at high risk for hypertension, stroke and possibly obesity. The mechanisms behind the association of intrauterine exposure to preeclampsia and high risk of health problems in the later life remain largely unknown. The aims of the current investigation were to determine the changes in DNA methylation at IGF2 and GNAS DMR in offspring of preeclamptic pregnancy and to explore the possible mechanisms underlying the association between maternal preeclampsia and high risk for health problems in the later life of their offspring. Results Umbilical cord blood was taken from infants born to women of preeclampsia (n=56), gestational hypertension (n=23) and normal pregnancy (n=81). DNA methylation levels of IGF2 and GNAS DMR were determined by Massarray quantitative methylation analysis. Methylation levels at IGF2 DMR were significantly lower in preeclampsia than normal pregnancy. The average methylation level at IGF2 DMR was significantly correlated with preeclampsia even after birth weight, maternal age, gestational age at delivery and fetal gender were adjusted. The difference in methylation level was not significantly different between mild and severe preeclampsia. The methylation level at GNAS DMR was not significantly correlated with birth weight, maternal age, gestational age at delivery, fetal gender, preeclampsia or gestational hypertension. Conclusions We concluded preeclampsia induced a decrease in methylation level at IGF 2 DMR, and this might be among the mechanisms behind the association between intrauterine exposure to preeclampsia and high risk for metabolic diseases in the later life of the infants.
- Published
- 2013
4. Transcriptome analysis of leaves, roots and flowers of Panax notoginseng identifies genes involved in ginsenoside and alkaloid biosynthesis.
- Author
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Ming-Hua Liu, Bin-Rui Yang, Wai-Fung Cheung, Yi Yang, Kevin, He-Feng Zhou, Sui-Lam Kwok, Jamie, Guo-Cheng Liu, Xiao-Feng Li, Silin Zhong, Ming-Yuen Lee, Simon, and Kwok-Wing Tsui, Stephen
- Subjects
PANAX ,PLANT genomes ,BIOSYNTHESIS ,GENE expression in plants ,RNA sequencing ,NUCLEOTIDE sequencing ,CYTOCHROME P-450 ,GLYCOSYLTRANSFERASES - Abstract
Background: Panax notoginseng (Burk.) F.H. Chen is one ofthe most highly valued medicinal plants in the world. The major bioactive molecules are triterpene saponins, which are also known as ginsenosides. However, its large genome size has hindered the assembly of a draft genome by whole genome sequencing. Hence, genomic and transcriptomic details about P. notoginseng, especially its biosynthetic pathways and gene expression in different parts ofthe plant, have remained largely unknown until now. Results: In this study, RNA sequencing of three different P. notoginseng tissues was performed using next generation DNA sequencing. After assembling the high quality sequencing reads into 107,340 unigenes, biochemical pathways were predicted and 9,908 unigenes were assigned to 135 KEGG pathways. Among them, 270 unigenes were identified to be involved in triterpene saponin biosynthesis. In addition, 350 and 342 unigenes were predicted to encode cytochrome P450s and glycosyltransferases, respectively, based on the annotation results, some of which encode enzymes responsible for the conversion of the triterpene saponin backbone into different ginsenosides. In particular, one unigene predominately expressed in the root was annotated as CYP716A53v2, which probably participates in the formation of protopanaxatriol from protopanaxadiol in P. notoginseng. The differential expression of this gene was further confirmed by real-time PCR. Conclusions: We have established a global transcriptome dataset for P. notoginseng and provided additional genetic information for further genome-wide research and analyses. Candidate genes involved in ginsenoside biosynthesis, including putative cytochrome P450s and glycosyltransferases were obtained. The transcriptomes in different plant tissues also provide invaluable resources for future study ofthe differences in physiological processes and secondary metabolites in different parts of P. notoginseng. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
5. Construction and verification of the transcriptional regulatory response network of Streptococcus mutans upon treatment with the biofilm inhibitor carolacton.
- Author
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Sudhakar, Padhmanand, Reck, Michael, Wei Wang, He, Feng Q., Dobler, Irene W., and An-Ping Zeng
- Subjects
GENETIC transcription regulation ,GENETIC regulation ,STREPTOCOCCUS mutans ,BIOFILMS ,MOLECULAR structure of transcription factors ,PHYSIOLOGY - Abstract
Background Carolacton is a newly identified secondary metabolite causing altered cell morphology and death of Streptococcus mutans biofilm cells. To unravel key regulators mediating these effects, the transcriptional regulatory response network of S. mutans biofilms upon carolacton treatment was constructed and analyzed. A systems biological approach integrating timeresolved transcriptomic data, reverse engineering, transcription factor binding sites, and experimental validation was carried out. Results The co-expression response network constructed from transcriptomic data using the reverse engineering algorithm called the Trend Correlation method consisted of 8284 gene-pairs. The regulatory response network inferred by superimposing transcription factor binding site information into the co-expression network comprised 329 putative transcriptional regulatory interactions and could be classified into 27 sub-networks each co-regulated by a transcription factor. These sub-networks were significantly enriched with genes sharing common functions. The regulatory response network displayed global hierarchy and network motifs as observed in model organisms. The sub-networks modulated by the pyrimidine biosynthesis regulator PyrR, the glutamine synthetase repressor GlnR, the cysteine metabolism regulator CysR, global regulators CcpA and CodY and the two component system response regulators VicR and MbrC among others could putatively be related to the physiological effect of carolacton. The predicted interactions from the regulatory network between MbrC, known to be involved in cell envelope stress response, and the murMN-SMU_718c genes encoding peptidoglycan biosynthetic enzymes were experimentally confirmed using Electro Mobility Shift Assays. Furthermore, gene deletion mutants of five predicted key regulators from the response networks were constructed and their sensitivities towards carolacton were investigated. Deletion of cysR, the node having the highest connectivity among the regulators chosen from the regulatory network, resulted in a mutant which was insensitive to carolacton thus demonstrating not only the essentiality of cysR for the response of S. mutans biofilms to carolacton but also the relevance of the predicted network. Conclusion The network approach used in this study revealed important regulators and interactions as part of the response mechanisms of S. mutans biofilm cells to carolacton. It also opens a door for further studies into novel drug targets against streptococci. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
6. Transcriptome analysis of leaves, roots and flowers of Panax notoginseng identifies genes involved in ginsenoside and alkaloid biosynthesis.
- Author
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Liu MH, Yang BR, Cheung WF, Yang KY, Zhou HF, Kwok JS, Liu GC, Li XF, Zhong S, Lee SM, and Tsui SK
- Subjects
- Cytochrome P-450 Enzyme System metabolism, Flowers genetics, Flowers metabolism, Gene Expression Profiling methods, Glycosyltransferases metabolism, Panax notoginseng genetics, Plant Leaves genetics, Plant Leaves metabolism, Plant Proteins metabolism, Plant Roots genetics, Plant Roots metabolism, Sapogenins metabolism, Alkaloids biosynthesis, Ginsenosides biosynthesis, Panax notoginseng metabolism, Plant Proteins genetics
- Abstract
Background: Panax notoginseng (Burk.) F.H. Chen is one of the most highly valued medicinal plants in the world. The major bioactive molecules are triterpene saponins, which are also known as ginsenosides. However, its large genome size has hindered the assembly of a draft genome by whole genome sequencing. Hence, genomic and transcriptomic details about P. notoginseng, especially its biosynthetic pathways and gene expression in different parts of the plant, have remained largely unknown until now., Results: In this study, RNA sequencing of three different P. notoginseng tissues was performed using next generation DNA sequencing. After assembling the high quality sequencing reads into 107,340 unigenes, biochemical pathways were predicted and 9,908 unigenes were assigned to 135 KEGG pathways. Among them, 270 unigenes were identified to be involved in triterpene saponin biosynthesis. In addition, 350 and 342 unigenes were predicted to encode cytochrome P450s and glycosyltransferases, respectively, based on the annotation results, some of which encode enzymes responsible for the conversion of the triterpene saponin backbone into different ginsenosides. In particular, one unigene predominantly expressed in the root was annotated as CYP716A53v2, which probably participates in the formation of protopanaxatriol from protopanaxadiol in P. notoginseng. The differential expression of this gene was further confirmed by real-time PCR., Conclusions: We have established a global transcriptome dataset for P. notoginseng and provided additional genetic information for further genome-wide research and analyses. Candidate genes involved in ginsenoside biosynthesis, including putative cytochrome P450s and glycosyltransferases were obtained. The transcriptomes in different plant tissues also provide invaluable resources for future study of the differences in physiological processes and secondary metabolites in different parts of P. notoginseng.
- Published
- 2015
- Full Text
- View/download PDF
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