1. RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri
- Author
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Changqing Chang, Wang Jinpei, Liu Qiongguang, Deng Jiaru, Liao Jinxing, and Kai-Huai Li
- Subjects
Xanthomonas ,lcsh:QH426-470 ,lcsh:Biotechnology ,RNA-Seq ,Biology ,Pilus ,Xanthomonas citri ,Transcriptome ,03 medical and health sciences ,Membrane Lipids ,lcsh:TP248.13-248.65 ,Genetics ,Fatty acids ,Biofilm formation ,Unsaturated fatty acid ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Cold-Shock Response ,Gene Expression Profiling ,Biofilm ,Motility ,biology.organism_classification ,lcsh:Genetics ,Metabolism ,Flagella ,Citrus canker ,Low temperature stress ,Biotechnology ,Research Article - Abstract
Background Xanthomonas citri pv. citri (Xcc) is a citrus canker causing Gram-negative bacteria. Currently, little is known about the biological and molecular responses of Xcc to low temperatures. Results Results depicted that low temperature significantly reduced growth and increased biofilm formation and unsaturated fatty acid (UFA) ratio in Xcc. At low temperature Xcc formed branching structured motility. Global transcriptome analysis revealed that low temperature modulates multiple signaling networks and essential cellular processes such as carbon, nitrogen and fatty acid metabolism in Xcc. Differential expression of genes associated with type IV pilus system and pathogenesis are important cellular adaptive responses of Xcc to cold stress. Conclusions Study provides clear insights into biological characteristics and genome-wide transcriptional analysis based molecular mechanism of Xcc in response to low temperature.
- Published
- 2019