1. Liver transcriptome analysis reveals extensive transcriptional plasticity during acclimation to low salinity in <italic>Cynoglossus semilaevis</italic>.
- Author
-
Si, Yufeng, Wen, Haishen, Li, Yun, He, Feng, Li, Jifang, Li, Siping, and He, Huiwen
- Subjects
- *
SALINITY , *ABIOTIC stress , *CYNOGLOSSIDAE , *FISH genomes , *OSMOTIC pressure - Abstract
Background: Salinity is an important abiotic stress that influences the physiological and metabolic activity, reproduction, growth and development of marine fish. It has been suggested that half-smooth tongue sole (
Cynoglossus semilaevis ), a euryhaline fish species, uses a large amount of energy to maintain osmotic pressure balance when exposed to fluctuations in salinity. To delineate the molecular response ofC. semilaevis to different levels of salinity, we performed RNA-seq analysis of the liver to identify the genes and molecular and biological processes involved in responding to salinity changes. Results: The present study yielded 330.4 million clean reads, of which 83.9% were successfully mapped to the reference genome ofC. semilaevis . One hundred twenty-eight differentially expressed genes (DEGs), including 43 up-regulated genes and 85 down-regulated genes, were identified. These DEGs were highly represented in metabolic pathways, steroid biosynthesis, terpenoid backbone biosynthesis, butanoate metabolism, glycerolipid metabolism and the 2-oxocarboxylic acid metabolism pathway. In addition, genes involved in metabolism, osmoregulation and ion transport, signal transduction, immune response and stress response, and cytoskeleton remodeling were affected during acclimation to low salinity. Genesacat2 ,fdps ,hmgcr ,hmgcs1 ,mvk ,pmvk ,ebp ,lss ,dhcr7 , anddhcr24 were up-regulated andabat ,ddc ,acy1 were down-regulated in metabolic pathways. Genesaqp10 andslc6a6 were down-regulated in osmoregulation and ion transport. Genesabat ,fdps ,hmgcs1 ,mvk ,pmvk and dhcr7 were first reported to be associated with salinity adaptation in teleosts. Conclusions: Our results revealed that metabolic pathways, especially lipid metabolism were important for salinity adaptation. The candidate genes identified from this study provide a basis for further studies to investigate the molecular mechanism of salinity adaptation and transcriptional plasticity in marine fish. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF