1. In-solution Y-chromosome capture-enrichment on ancient DNA libraries
- Author
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Diana I. Cruz-Dávalos, María A. Nieves-Colón, Alexandra Sockell, G. David Poznik, Hannes Schroeder, Anne C. Stone, Carlos D. Bustamante, Anna-Sapfo Malaspinas, and María C. Ávila-Arcos
- Subjects
Ancient DNA ,Capture-enrichment ,Y chromosome ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach—in-solution capture-enrichment—retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC + YCC) versus non-enriched (pre-capture) libraries. Results The six samples show different levels of initial endogenous content, with very low (
- Published
- 2018
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