1. miRNeye: a microRNA expression atlas of the mouse eye
- Author
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Vincenzo A. Gennarino, Sandro Banfi, Marianthi Karali, Marchesa Bilio, Roberta Verde, Ivana Peluso, Pascal Dollé, Giampiero Lago, Karali, M, Peluso, I, Gennarino, Va, Bilio, M, Verde, R, Lago, G, Dollé, P, Banfi, Sandro, Telethon Institute for Genetics and Medicine, Telethon Institute, Department of Molecular and Human Genetics, Baylor College of Medicine (BCM), Baylor University-Baylor University, Istituto di Chimica Biomolecolare (ICB), Centre National de la Recherche (CNR), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the European Community (EURExpress grant n. LSHG-CT-2004- 512003 and EVI-GENORET grant n. LSHG-CT-2005-512036) and the Fondazione Telethon. MK acknowledges financial support by a Marie Curie European Re-integration Grant (grant n. PERG03-GA-2008-231068)., and BMC, Ed.
- Subjects
Time Factors ,Microarray ,genetic structures ,Iris ,Retinal Pigment Epithelium ,Proteomics ,Eye ,Cornea ,Mice ,0302 clinical medicine ,MESH: Gene Expression Regulation, Developmental ,Databases, Genetic ,MESH: Animals ,MESH: Organ Specificity ,MESH: Databases, Genetic ,In Situ Hybridization ,Genetics ,Regulation of gene expression ,0303 health sciences ,Gene Expression Regulation, Developmental ,MESH: Lens, Crystalline ,MESH: Retinal Pigment Epithelium ,medicine.anatomical_structure ,MESH: Internet ,Organ Specificity ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,DNA microarray ,Biotechnology ,Research Article ,lcsh:QH426-470 ,lcsh:Biotechnology ,MESH: Eye ,In situ hybridization ,Computational biology ,Biology ,MESH: Ciliary Body ,03 medical and health sciences ,MESH: Gene Expression Profiling ,MESH: In Situ Hybridization ,MESH: Mice, Inbred C57BL ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,lcsh:TP248.13-248.65 ,microRNA ,Lens, Crystalline ,medicine ,Animals ,MESH: Mice ,030304 developmental biology ,Internet ,Retinal pigment epithelium ,Gene Expression Profiling ,MESH: Time Factors ,MESH: Cornea ,Ciliary Body ,MESH: Iris ,eye diseases ,Gene expression profiling ,Mice, Inbred C57BL ,MicroRNAs ,lcsh:Genetics ,030221 ophthalmology & optometry ,sense organs ,MESH: MicroRNAs - Abstract
Background MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. Results In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. Conclusions This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
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- 2010