1. Genome-wide identification and functional prediction of novel and fungi-responsive lincRNAs in Triticum aestivum
- Author
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Shikai Lv, Changyou Wang, Hong Zhang, Xinlun Liu, Wanquan Ji, Jilei Hao, Yanzhen Wang, Yajuan Wang, Weiguo Hu, and Wei Tong
- Subjects
0301 basic medicine ,Blumeria graminis ,RNA-Seq ,Stripe rust ,Genome ,Transcriptome ,03 medical and health sciences ,Intergenic region ,Powdery mildew ,Ascomycota ,Botany ,Genetics ,snRNP ,Triticum ,Plant Diseases ,biology ,miRNA target ,Basidiomycota ,food and beverages ,Chromosome Mapping ,biology.organism_classification ,MicroRNAs ,030104 developmental biology ,RNA, Plant ,Wheat ,lincRNA ,Sm-site ,Host-Pathogen Interactions ,RNA, Long Noncoding ,DNA microarray ,Biotechnology ,Research Article - Abstract
Background Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Increasingly evidences suggest that long intergenic ncRNAs (lincRNAs) are developmentally regulated and play important roles in development and stress responses of plants. However, identification of lincRNAs in wheat is still limited comparing with functional gene expression. Results The transcriptome of the hexaploid wheat line N9134 inoculated with the Chinese Pst race CYR31 and Bgt race E09 at 1, 2, and 3 days post-inoculation was recapitulated to detect the lincRNAs. Here, 283 differential expressed lincRNAs were identified from 58218 putative lincRNAs, which account for 31.2 % of transcriptome. Of which, 254 DE-LincRNAs responded to the Bgt stress, and 52 lincRNAs in Pst. Among them, 1328 SnRNP motifs (sm sites) were detected and showed RRU4–11RR sm site element and consensus RRU1–9VU1–7RR SnRNP motifs, where the total number of uridine was more than 3 but less than 11. Additionally, 101 DE-lincRNAs were predicted as targets of miRNA by psRNATarget, while 5 target mimics were identified using target mimicry search in TAPIR. Conclusions Taken together, our findings indicate that the lincRNA of wheat responded to Bgt and Pst stress and played important roles in splicesome and inter-regulating with miRNA. The sm site of wheat showed a more complex construction than that in mammal and model plant. The mass sequence data generated in this study provide a cue for future functional and molecular research on wheat–fungus interactions. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2570-0) contains supplementary material, which is available to authorized users.
- Published
- 2016