1. Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose
- Author
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Yupu Song, Chang Liu, Yuxuan Zhou, Guangyu Lin, Chenguang Xu, Petunia Msuthwana, Sihui Wang, Jingyun Ma, Fangming Zhuang, Xianou Fu, Yudong Wang, Tuoya Liu, Qianyan Liu, Jingbo Wang, Yujian Sui, and Yongfeng Sun
- Subjects
Hungarian white goose ,Feather follicle ,Goose down ,SNP ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Hungarian white goose has excellent down production performance and was introduced to China in 2010. The growth and development of feather follicles has an important impact on down production. Goose feather follicles can be divided into primary and secondary feather follicles, both of which originate in the embryonic stage. Msx2 (Msh Homeobox 2) plays a regulatory role in tissues and organs such as eyes, teeth, bones and skin. However, its regulatory mechanism on goose feather follicles development remains unclear. Results Msx2 gene first increased, then decreased and increased at the end (E13, E18, E23, E28) during embryonic feather follicle development, and the expression level was the highest at E18. The pEGFP-N1-Msx2 overexpression vector and si-Msx2 siRNA vector were constructed to transfect goose embryo dermal fibroblasts. The results showed that the cell viability of ov-Msx2 group was significantly increased, and the gene expression levels of FGF5 and TGF-β1 genes were significantly down-regulated (P
- Published
- 2022
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