1. Utility of paired plasma and drainage fluid mNGS in diagnosing acute intra-abdominal infections with sepsis
- Author
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Jia-yu Mao, Dong-kai Li, Dong Zhang, Qi-wen Yang, Yun Long, and Na Cui
- Subjects
Sepsis ,Acute intra-abdominal infection ,mNGS ,Plasma and peritoneal drainage ,Antibiotics ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Metagenomic next-generation sequencing (mNGS) has been increasingly applied in sepsis. We aimed to evaluate the diagnostic and therapeutic utility of mNGS of paired plasma and peritoneal drainage (PD) fluid samples in comparison to culture-based microbiological tests (CMTs) among critically ill patients with suspected acute intra-abdominal infections (IAIs). Methods We conducted a prospective study from October 2021 to December 2022 enrolling septic patients with suspected IAIs (n = 111). Pairwise CMTs and mNGS of plasma and PD fluid were sent for pathogen detection. The mNGS group underwent therapeutic regimen adjustment based on mNGS results for better treatment. The microbial community structure, clinical features, antibiotic use and prognoses of the patients were analyzed. Results Higher positivity rates were observed with mNGS versus CMTs for both PD fluid (90.0% vs. 48.3%, p
- Published
- 2024
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