9 results on '"Basnyat, Buddha"'
Search Results
2. Melioidosis: misdiagnosed in Nepal
- Author
-
Shrestha, Neha, Adhikari, Mahesh, Pant, Vivek, Baral, Suman, Shrestha, Anjan, Basnyat, Buddha, Sharma, Sangita, and Sherchand, Jeevan Bahadur
- Published
- 2019
- Full Text
- View/download PDF
3. The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens
- Author
-
Chau Tran, Campbell James I, Tu Le, Holt Kathryn, Dunstan Sarah, Thuy Hang, Dongol Sabina, Karkey Abhilasha, Nga Tran, Chau Nguyen, Arjyal Amit, Koirala Samir, Basnyat Buddha, Dolecek Christiane, Farrar Jeremy, and Baker Stephen
- Subjects
Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background PCR amplification for the detection of pathogens in biological material is generally considered a rapid and informative diagnostic technique. Invasive Salmonella serovars, which cause enteric fever, can be commonly cultured from the blood of infected patients. Yet, the isolation of invasive Salmonella serovars from blood is protracted and potentially insensitive. Methods We developed and optimised a novel multiplex three colour real-time PCR assay to detect specific target sequences in the genomes of Salmonella serovars Typhi and Paratyphi A. We performed the assay on DNA extracted from blood and bone marrow samples from culture positive and negative enteric fever patients. Results The assay was validated and demonstrated a high level of specificity and reproducibility under experimental conditions. All bone marrow samples tested positive for Salmonella, however, the sensitivity on blood samples was limited. The assay demonstrated an overall specificity of 100% (75/75) and sensitivity of 53.9% (69/128) on all biological samples. We then tested the PCR detection limit by performing bacterial counts after inoculation into blood culture bottles. Conclusions Our findings corroborate previous clinical findings, whereby the bacterial load of S. Typhi in peripheral blood is low, often below detection by culture and, consequently, below detection by PCR. Whilst the assay may be utilised for environmental sampling or on differing biological samples, our data suggest that PCR performed directly on blood samples may be an unsuitable methodology and a potentially unachievable target for the routine diagnosis of enteric fever.
- Published
- 2010
- Full Text
- View/download PDF
4. High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children
- Author
-
Parkhill Julian, Song Yajun, Pulickal Anoop S, Thorson Stephen, Adhikari Neelam, Basnyat Buddha, Dongol Sabina, Baker Stephen, Holt Kathryn E, Farrar Jeremy J, Murdoch David R, Kelly Dominic F, Pollard Andrew J, and Dougan Gordon
- Subjects
Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Salmonella Typhi (S. Typhi) causes typhoid fever, which remains an important public health issue in many developing countries. Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection. Methods We recently defined the population structure of S. Typhi, using new sequencing technologies to identify nearly 2,000 single nucleotide polymorphisms (SNPs) that can be used as unequivocal phylogenetic markers. Here we have used the GoldenGate (Illumina) platform to simultaneously type 1,500 of these SNPs in 62 S. Typhi isolates causing severe typhoid in children admitted to Patan Hospital in Kathmandu. Results Eight distinct S. Typhi haplotypes were identified during the 20-month study period, with 68% of isolates belonging to a subclone of the previously defined H58 S. Typhi. This subclone was closely associated with resistance to nalidixic acid, with all isolates from this group demonstrating a resistant phenotype and harbouring the same resistance-associated SNP in GyrA (Phe83). A secondary clone, comprising 19% of isolates, was observed only during the second half of the study. Conclusions Our data demonstrate the utility of SNP typing for monitoring bacterial populations over a defined period in a single endemic setting. We provide evidence for genotype introduction and define a nalidixic acid resistant subclone of S. Typhi, which appears to be the dominant cause of severe pediatric typhoid in Kathmandu during the study period.
- Published
- 2010
- Full Text
- View/download PDF
5. High-throughput bacterial SNP typing identifies distinct clusters of SalmonellaTyphi causing typhoid in Nepalese children
- Author
-
Holt, Kathryn E, primary, Baker, Stephen, additional, Dongol, Sabina, additional, Basnyat, Buddha, additional, Adhikari, Neelam, additional, Thorson, Stephen, additional, Pulickal, Anoop S, additional, Song, Yajun, additional, Parkhill, Julian, additional, Farrar, Jeremy J, additional, Murdoch, David R, additional, Kelly, Dominic F, additional, Pollard, Andrew J, additional, and Dougan, Gordon, additional
- Published
- 2010
- Full Text
- View/download PDF
6. The sensitivity of real-time PCR amplification targeting invasive Salmonellaserovars in biological specimens
- Author
-
Nga, Tran Vu Thieu, primary, Karkey, Abhilasha, additional, Dongol, Sabina, additional, Thuy, Hang Nguyen, additional, Dunstan, Sarah, additional, Holt, Kathryn, additional, Tu, Le Thi Phuong, additional, Campbell, James I, additional, Chau, Tran Thuy, additional, Chau, Nguyen Van Vinh, additional, Arjyal, Amit, additional, Koirala, Samir, additional, Basnyat, Buddha, additional, Dolecek, Christiane, additional, Farrar, Jeremy, additional, and Baker, Stephen, additional
- Published
- 2010
- Full Text
- View/download PDF
7. High-throughput bacterial SNP typing identifiesdistinct clusters of Salmonella Typhi causingtyphoid in Nepalese children.
- Author
-
Holt, Kathryn E., Baker, Stephen, Dongol, Sabina, Basnyat, Buddha, Adhikari, Neelam, Thorson, Stephen, Pulickal, Anoop S., Yajun Song, Parkhill, Julian, Farrar, Jeremy J., Murdoch, David R., Kelly, Dominic F., Pollard, Andrew J., and Dougan, Gordon
- Subjects
SALMONELLA typhi ,TYPHOID fever ,PUBLIC health ,BIOMARKERS - Abstract
Background: Salmonella Typhi (S. Typhi) causes typhoid fever, which remains an important public health issue in many developing countries. Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection. Methods: We recently defined the population structure of S. Typhi, using new sequencing technologies to identify nearly 2,000 single nucleotide polymorphisms (SNPs) that can be used as unequivocal phylogenetic markers. Here we have used the GoldenGate (Illumina) platform to simultaneously type 1,500 of these SNPs in 62 S. Typhi isolates causing severe typhoid in children admitted to Patan Hospital in Kathmandu. Results: Eight distinct S. Typhi haplotypes were identified during the 20-month study period, with 68% of isolates belonging to a subclone of the previously defined H58 S. Typhi. This subclone was closely associated with resistance to nalidixic acid, with all isolates from this group demonstrating a resistant phenotype and harbouring the same resistance-associated SNP in GyrA (Phe83). A secondary clone, comprising 19% of isolates, was observed only during the second half of the study. Conclusions: Our data demonstrate the utility of SNP typing for monitoring bacterial populations over a defined period in a single endemic setting. We provide evidence for genotype introduction and define a nalidixic acid resistant subclone of S. Typhi, which appears to be the dominant cause of severe pediatric typhoid in Kathmandu during the study period. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
8. The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens.
- Author
-
Nga, Tran Vu Thieu, Karkey, Abhilasha, Dongol, Sabina, Thuy, Hang Nguyen, Dunstan, Sarah, Holt, Kathryn, Tu, Le Thi Phuong, Campbell, James I., Chau, Tran Thuy, Chau, Nguyen Van Vinh, Arjyal, Amit, Koirala, Samir, Basnyat, Buddha, Dolecek, Christiane, Farrar, Jeremy, and Baker, Stephen
- Subjects
TYPHOID fever ,SALMONELLA diseases ,POLYMERASE chain reaction ,PATHOGENIC microorganisms ,BIOMEDICAL materials - Abstract
Background: PCR amplification for the detection of pathogens in biological material is generally considered a rapid and informative diagnostic technique. Invasive Salmonella serovars, which cause enteric fever, can be commonly cultured from the blood of infected patients. Yet, the isolation of invasive Salmonella serovars from blood is protracted and potentially insensitive. Methods: We developed and optimised a novel multiplex three colour real-time PCR assay to detect specific target sequences in the genomes of Salmonella serovars Typhi and Paratyphi A. We performed the assay on DNA extracted from blood and bone marrow samples from culture positive and negative enteric fever patients. Results: The assay was validated and demonstrated a high level of specificity and reproducibility under experimental conditions. All bone marrow samples tested positive for Salmonella, however, the sensitivity on blood samples was limited. The assay demonstrated an overall specificity of 100% (75/75) and sensitivity of 53.9% (69/128) on all biological samples. We then tested the PCR detection limit by performing bacterial counts after inoculation into blood culture bottles. Conclusions: Our findings corroborate previous clinical findings, whereby the bacterial load of S. Typhi in peripheral blood is low, often below detection by culture and, consequently, below detection by PCR. Whilst the assay may be utilised for environmental sampling or on differing biological samples, our data suggest that PCR performed directly on blood samples may be an unsuitable methodology and a potentially unachievable target for the routine diagnosis of enteric fever. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
9. High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children.
- Author
-
Holt KE, Baker S, Dongol S, Basnyat B, Adhikari N, Thorson S, Pulickal AS, Song Y, Parkhill J, Farrar JJ, Murdoch DR, Kelly DF, Pollard AJ, and Dougan G
- Subjects
- Bacterial Proteins genetics, Child, Child, Preschool, Cluster Analysis, DNA Gyrase genetics, Drug Resistance, Bacterial, Female, Genotype, Haplotypes, High-Throughput Screening Assays, Humans, Infant, Male, Molecular Epidemiology, Nepal epidemiology, Salmonella typhi classification, Salmonella typhi isolation & purification, Bacterial Typing Techniques, DNA, Bacterial genetics, Polymorphism, Single Nucleotide, Salmonella typhi genetics, Typhoid Fever epidemiology, Typhoid Fever microbiology
- Abstract
Background: Salmonella Typhi (S. Typhi) causes typhoid fever, which remains an important public health issue in many developing countries. Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection., Methods: We recently defined the population structure of S. Typhi, using new sequencing technologies to identify nearly 2,000 single nucleotide polymorphisms (SNPs) that can be used as unequivocal phylogenetic markers. Here we have used the GoldenGate (Illumina) platform to simultaneously type 1,500 of these SNPs in 62 S. Typhi isolates causing severe typhoid in children admitted to Patan Hospital in Kathmandu., Results: Eight distinct S. Typhi haplotypes were identified during the 20-month study period, with 68% of isolates belonging to a subclone of the previously defined H58 S. Typhi. This subclone was closely associated with resistance to nalidixic acid, with all isolates from this group demonstrating a resistant phenotype and harbouring the same resistance-associated SNP in GyrA (Phe83). A secondary clone, comprising 19% of isolates, was observed only during the second half of the study., Conclusions: Our data demonstrate the utility of SNP typing for monitoring bacterial populations over a defined period in a single endemic setting. We provide evidence for genotype introduction and define a nalidixic acid resistant subclone of S. Typhi, which appears to be the dominant cause of severe pediatric typhoid in Kathmandu during the study period.
- Published
- 2010
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.