Search

Your search keyword '"Aili Cui"' showing total 2 results
Start Over Author "Aili Cui" Journal bmc infectious diseases
2 results on '"Aili Cui"'

1. Nationwide distribution of varicella-zoster virus clades in China.

Author

Songtao Xu, Mukai Chen, Huanying Zheng, Haiyan Wang, Meng Chen, Jianhui Zhou, Wang Shuang, Pengbo Yu, Chaofeng Ma, Jilan He, Daxing Feng, Zhu Zhen, Zhang Yan, Mao Naiying, Aili Cui, Qiuhua Wu, Mengyuan Qi, Chongshan Li, Xiaoguang Xu, and Wenbo Xu

Subjects
VARICELLA-zoster virus, ENDONUCLEASES, SINGLE nucleotide polymorphisms, RESTRICTION fragment length polymorphisms, GENE mapping, CHICKENPOX vaccines
Abstract

Background: In 2010, a universal nomenclature for varicella-zoster virus (VZV) clades was established, which is very useful in the monitoring of viral evolution, recombination, spread and genetic diversity. Currently, information about VZV clades has been disclosed worldwide, however, there are limited data regarding the characterization of circulating VZV clades in China, even where varicella remains widely epidemic. Methods: From 2008 to 2012, clinical samples with varicella or zoster were collected in General Hospital in eight provinces and analyzed by PCR, restriction endonuclease digestion and sequencing. The viral clades were determined by analysis of five single nucleotide polymorphisms (SNPs) within the 447-bp fragment of open reading frame (ORF) 22, and the restriction fragment length polymorphisms (RFLPs) of ORF 38 (PstI), ORF 54 (BglI) and ORF 62 (SmaI) were evaluated to understand genetic diversity of VZV and determinate varicella vaccine adverse event (VVAE). Results: Seventy-seven varicella and 11 zoster samples were identified as being positive for VZV. The five SNPs profile showed that the majority of VZV strains belonged to clade 2, but clade 5 and clade 4 strains were also found in Guangdong. The RFLPs analysis of the DNA fragments of ORF 38, 54 and 62 showed that 85 of these samples were characterized as PstI + BglI + SamI-, and the remaining three VZV strains from varicella patients were characterized as PstI-BglI + SamI+ which is the genetic profile of VVAEs. Conclusions: The study suggested that the predominant clade 2 VZVs had been continually circulating since at least the 1950s in China. Nearly all VZV strains except VVAEs possessed the genetic profile of PstI + BglI + Sam-. However, the other clades were also found to be co-circulating with clade 2, especially in the border regions. These results highlighted the need for the constant and broad use of virologic surveillance to provide an important genetic baseline for varicella control and vaccination programs in China. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

2. Nationwide distribution of varicella-zoster virus clades in China

Author

Zhu Zhen, Mengyuan Qi, Qiuhua Wu, Wang Shuang, Huanying Zheng, Xiaoguang Xu, Zhang Yan, Meng Chen, Wenbo Xu, Pengbo Yu, Mukai Chen, Songtao Xu, Haiyan Wang, Chaofeng Ma, Daxing Feng, Jilan He, Jianhui Zhou, Aili Cui, Mao Nai-ying, and Chongshan Li

Subjects
Adult, Clade, Genetic profile, 0301 basic medicine, China, Herpesvirus 3, Human, Adolescent, Genotype, Varicella vaccine, viruses, Biology, Hospitals, General, medicine.disease_cause, Herpes Zoster, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Virus, SmaI, Chickenpox Vaccine, Open Reading Frames, Young Adult, 03 medical and health sciences, Chickenpox, medicine, Humans, Child, Aged, integumentary system, Varicella zoster virus, virus diseases, Middle Aged, biochemical phenomena, metabolism, and nutrition, Biological Evolution, Virology, Restriction enzyme, 030104 developmental biology, Infectious Diseases, Child, Preschool, Viral evolution, Varicella-zoster virus, Varicella vaccine adverse event, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length, Research Article
Abstract

Background In 2010, a universal nomenclature for varicella-zoster virus (VZV) clades was established, which is very useful in the monitoring of viral evolution, recombination, spread and genetic diversity. Currently, information about VZV clades has been disclosed worldwide, however, there are limited data regarding the characterization of circulating VZV clades in China, even where varicella remains widely epidemic. Methods From 2008 to 2012, clinical samples with varicella or zoster were collected in General Hospital in eight provinces and analyzed by PCR, restriction endonuclease digestion and sequencing. The viral clades were determined by analysis of five single nucleotide polymorphisms (SNPs) within the 447-bp fragment of open reading frame (ORF) 22, and the restriction fragment length polymorphisms (RFLPs) of ORF 38 (PstI), ORF 54 (BglI) and ORF 62 (SmaI) were evaluated to understand genetic diversity of VZV and determinate varicella vaccine adverse event (VVAE). Results Seventy-seven varicella and 11 zoster samples were identified as being positive for VZV. The five SNPs profile showed that the majority of VZV strains belonged to clade 2, but clade 5 and clade 4 strains were also found in Guangdong. The RFLPs analysis of the DNA fragments of ORF 38, 54 and 62 showed that 85 of these samples were characterized as PstI + BglI + SamI-, and the remaining three VZV strains from varicella patients were characterized as PstI-BglI + SamI+ which is the genetic profile of VVAEs. Conclusions The study suggested that the predominant clade 2 VZVs had been continually circulating since at least the 1950s in China. Nearly all VZV strains except VVAEs possessed the genetic profile of PstI + BglI + Sam-. However, the other clades were also found to be co-circulating with clade 2, especially in the border regions. These results highlighted the need for the constant and broad use of virologic surveillance to provide an important genetic baseline for varicella control and vaccination programs in China.

Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results