1. Development of a sensitive novel diagnostic kit for the highly pathogenic avian influenza A (H5N1) virus
- Author
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Yasuko Tsunetsugu-Yokota, Hang L.K. Nguyen, Kazuo Ohnishi, Mai T.Q. Le, Giang T. Dang, Kengo Nishimura, Long T. Nguyen, Shigeyuki Itamura, Mie Kobayashi-Ishihara, Shuhei Misawa, Hitoshi Takahashi, Ikuyo Takayama, Tsutomu Kageyama, and Masato Tashiro
- Subjects
Monoclonal antibody ,medicine.medical_specialty ,medicine.drug_class ,viruses ,Hemagglutinin (influenza) ,Clinical specimens ,medicine.disease_cause ,Sensitivity and Specificity ,Virus ,Rapid influenza diagnosis ,Immunoenzyme Techniques ,Medical microbiology ,Influenza, Human ,Pandemic ,medicine ,Humans ,H5 hemagglutinin ,Influenza A Virus, H5N1 Subtype ,medicine.diagnostic_test ,biology ,business.industry ,Highly pathogenic avian influenza virus ,virus diseases ,Virology ,Influenza A virus subtype H5N1 ,Infectious Diseases ,Vietnam ,Parasitology ,Immunoassay ,biology.protein ,Pharynx ,Reagent Kits, Diagnostic ,business ,Research Article - Abstract
Background Sporadic emergence of the highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is a serious concern because of the potential for a pandemic. Conventional or quantitative RT-PCR is the standard laboratory test to detect viral influenza infections. However, this technology requires well-equipped laboratories and highly trained personnel. A rapid, sensitive, and specific alternative screening method is needed. Methods By a luminescence-linked enzyme immunoassay, we have developed a H5N1 HPAI virus detection kit using anti-H5 hemagglutinin monoclonal antibodies in combination with the detection of a universal NP antigen of the type A influenza virus. The process takes 15 minutes by use of the fully automated luminescence analyzer, POCube. Resutls We tested this H5/A kit using 19 clinical specimens from 13 patients stored in Vietnam who were infected with clade 1.1 or clade 2.3.4 H5N1 HPAI virus. Approximately 80% of clinical specimens were H5-positive using the POCube system, whereas only 10% of the H5-positive samples were detected as influenza A-positive by an immunochromatography-based rapid diagnostic kit. Conclusions This novel H5/A kit using POCube is served as a rapid and sensitive screening test for H5N1 HPAI virus infection in humans.
- Published
- 2014
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