1. Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?
- Author
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Shan-mei Wang, Dacheng Guo, Zeyu Wang, and Yu Xi
- Subjects
0301 basic medicine ,Gram-positive bacteria ,030106 microbiology ,Pcr assay ,medicine.disease_cause ,DNA, Ribosomal ,Polymerase Chain Reaction ,Sensitivity and Specificity ,DNA sequencing ,CAMP test ,Streptococcus agalactiae ,lcsh:Infectious and parasitic diseases ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Streptococcal Infections ,medicine ,Humans ,Standard test ,lcsh:RC109-216 ,030212 general & internal medicine ,Bacteriological Techniques ,biology ,biology.organism_classification ,16S ribosomal RNA ,PCR ,Infectious Diseases ,Parasitology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Vagina ,Female ,16S rDNA gene sequencing ,Research Article - Abstract
Background For a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough. Methods To confirm whether a positive CAMP test is a requirement for the identification of S.agalactiae, five suspected CAMP-negative S.agalactiae isolates from two hospitals, confirmed as Gram-positive and catalase-negative streptococci, were verified by the CAMP test in three batches of plates from two manufacturers and identified by the Phoenix system, MALDI-TOF MS, the PCR assay and the 16S rDNA gene sequencing. Results All five suspected strains were S.agalactiae, four of which were CAMP-negative and one of which was not S.agalactiae by the PCR assay. Conclusions A positive CAMP test was lacking sensitivity for the identification of S.agalactiae, and the question of whether the cfb gene is worthy of targeting should be further studied.
- Published
- 2019