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3. Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae

Author

Hassan Amini, Aref Delkhosh, Reza Rahbarghazi, Mehdi Hassanpour, Morteza Heidarzadeh, Shirin Saberianpour, Emel Sokullu, Heidarzadeh, Morteza, Sokullu, Emel (ORCID 0000-0003-1302-1997 & YÖK ID 163024), Rahbarghazi, Reza, Saberianpour, Shirin, Delkhosh, Aref, Amini, Hassan, Hassanpour, Mehdi, Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM), Graduate School of Health Sciences, and School of Medicine

Subjects
0301 basic medicine, Survival rate, Endothelial cells, Cell, lcsh:Medicine, Attachment, 02 engineering and technology, General Biochemistry, Genetics and Molecular Biology, Umbilical vein, Life sciences, Biomedicine, Science and technology, Biology, Multidisciplinary sciences, Ovine aorta, 03 medical and health sciences, chemistry.chemical_compound, Gentamicin protection assay, medicine, Animals, Humans, DAPI, lcsh:Science (General), lcsh:QH301-705.5, Aorta, chemistry.chemical_classification, Decellularization, Acellularization, Sheep, biology, Tissue Engineering, lcsh:R, General Medicine, 021001 nanoscience & nanotechnology, Molecular biology, Staining, Research Note, 030104 developmental biology, medicine.anatomical_structure, Enzyme, chemistry, lcsh:Biology (General), biology.protein, Collagen, 0210 nano-technology, Elastin, lcsh:Q1-390
Abstract

Objective: the current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, 1% Tween-20, and 1% SDS in combination with Trypsin–EDTA on acellularization of ovine aortae after 7 days. Results: Hematoxylin–Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p < 0.05). SEM imaging showed flattening of endothelial cells on the acellular surface., Tabriz University of Medical Sciences

Published
2020

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