1. Effect of heat inactivation and bulk lysis on real-time reverse transcription PCR detection of the SARS-COV-2: an experimental study.
- Author
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Leta D, Gutema G, Hagos GG, Diriba R, Bulti G, Sura T, Ayana D, Chala D, Lenjiso B, Bulti J, Abdella S, and Tola HH
- Subjects
- COVID-19 Testing, Hot Temperature, Humans, Real-Time Polymerase Chain Reaction, SARS-CoV-2 genetics, COVID-19 diagnosis, Reverse Transcription
- Abstract
Objective: This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection., Results: About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing., (© 2022. The Author(s).)
- Published
- 2022
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