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Start Over Author "N. Gibelli" Journal bollettino della societa italiana di biologia sperimentale
2 results on '"N. Gibelli"'

1. [Qualitative and quantitative histochemical evaluation of the dehydrogenase activity in human glioblastoma cells treated with beta-interferon]

Author

R, Nano, R, Rezzani, C, Zibera, N, Gibelli, and G, Bacciocchi

Subjects
Brain Neoplasms, Depression, Chemical, Enzyme Induction, Interferon Type I, Tumor Cells, Cultured, Humans, Glioma, Oxidoreductases, Cell Line, Neoplasm Proteins
Abstract

In an attempt to clarify the role of beta interferon in vitro cell systems, the metabolic expression of dihydrofolate reductase (DHFR), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), by histochemical methods was studied. DHFR was also quantified by flow cytometry. Glioblastoma cell line with or without beta interferon was used. Three days after the treatment the DHFR reaction was much less intense than in the control. Quantitative data confirmed these results. Immediately afterwards, most cells exhibited much more intense reaction. These facts underlined that, in this biological model in vitro, beta interferon could reduce the synthesis of these enzymes only for a short period.

Published
1990

2. [Qualitative and quantitative histochemical evaluation of the dehydrogenase activity in human glioblastoma cells treated with beta-interferon].

Author

Nano R, Rezzani R, Zibera C, Gibelli N, and Bacciocchi G

Subjects
Brain Neoplasms enzymology, Cell Line, Depression, Chemical, Enzyme Induction drug effects, Glioma enzymology, Humans, Tumor Cells, Cultured drug effects, Tumor Cells, Cultured enzymology, Brain Neoplasms pathology, Glioma pathology, Interferon Type I pharmacology, Neoplasm Proteins analysis, Oxidoreductases analysis
Abstract

In an attempt to clarify the role of beta interferon in vitro cell systems, the metabolic expression of dihydrofolate reductase (DHFR), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), by histochemical methods was studied. DHFR was also quantified by flow cytometry. Glioblastoma cell line with or without beta interferon was used. Three days after the treatment the DHFR reaction was much less intense than in the control. Quantitative data confirmed these results. Immediately afterwards, most cells exhibited much more intense reaction. These facts underlined that, in this biological model in vitro, beta interferon could reduce the synthesis of these enzymes only for a short period.

Published
1990

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