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Cultured fibroblast derived from 7 and 14 days chick embryonic skin were tested with three fluorescent lectins (Con A, WGA and SBA). The result obtained shown that the percentage of cells that bind WGA decrease from 7 to 14 days, supporting the presence of an age-dependent heterogeneity at cell surface level. In both cellular population only the 50% of the cell bind to SBA, suggesting a more subtle heterogeneity into the same population.

This paper shows that cultured chick embryo fibroblasts derived from lung, heart and skin contain different amounts of sulphurated glycosaminoglycans (GAG). The sulphurated GAGs were detected by X-ray microanalysis at scanning electron microscopy. The results support the current hypothesis on the heterogeneity of the embryonic fibroblast populations.

The aim of our study is to evaluate the eventual activity of the total dialysate of two uremic nephrectomized patients in recirculating dialysis and the chromatographic peak of the dialysate fractionated by Sephadex column G 15 on PMN chemotaxis. Only the total dialysate and the chromatographic peak B showed inhibition of chemotaxis. On the contrary the commercial polyamines in the same concentration range, and the other chromatographic peaks, containing polyamines too, did not revealed inhibition. Our data show, therefore, that the chemotaxis inhibition could be due to the middle-molecules present in the peak B, rather than the polyamines itself. Polyamines were determined by dansylation method, separated by thin layer chromatography and quantified by spectrofluorimeter. Chemotaxis was evaluated using the modified Boyden chamber.

We studied possible effects of uremic toxins and polyamines (PAs) on organ cultures of chick embryo. We added on culture media the lyophilized of total dialysate and its chromatographic peak II obtained with chromatography with Sephadex G 15. The total dialysate and peak II showed toxicity with degeneration of the culture, whereas the free PAs, we added, did not effects.