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Osteoprotegerin gene (OPG) is an important candidate gene of osteoporosis. Association of the OPG polymorphisms and bone mineral density (BMD) have been studied by several research groups, however results are not uniform. The aim of this study was to determine if two polymorphisms in the OPG gene influence bone turnover markers and bone mineral density (BMD) in postmenopausal women and elderly men. A total of 135 patients, aged 41–87 years, were included in this study. Lumbar spine, femoral neck, total-hip and distal radius BMD were measured by dual-energy X-ray absorptiometry (DXA) and bone turnover markers were measured by standard biochemical procedures. OPG gene polymorphisms A163OG and T245OG were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The frequencies of A163OG and T245OG polymorphisms in the OPG gene were determined by screening 131 DNA samples. The prevalence of genotypes of the A163G polymorphism was 59.4% for GG, 33.3% for AG and 7.2% for AA genotype in group with osteoporosis, whereas in control group the prevalence was 77.8, 16.7 and 5.6%, respectively. The prevalence of genotypes of the T245G polymorphism was 88.4% for genotype TT and 11.6% for genotype TG in group with osteoporosis, whereas in control group the prevalence was 94.4 and 5.6%, respectively. Analysis of BMD in the distal radius of postmenopausal women showed a trend to lower levels in the minor allele homozygote group (GG) vs two other two groups. Conclusion Our results suggest that OPG polymorphism influence BMD in postmenopausal women, however further biological and/or functional evidence would be needed to confirm the suggestive influence of OPG polymorphisms on BMD.