1. Analysis of peripheral blood progenitor cells demonstrates limitations of minimal residual disease diagnosis in a case of acute promyelocytic leukemia.
- Author
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Karlic HI, Mühlberger H, Pavlova BG, Pfeilstöcker M, Salamon J, Pittermann E, Heinz R, and Koller E
- Subjects
- Adult, Base Sequence, Bone Marrow pathology, Humans, Immunophenotyping, Leukemia, Promyelocytic, Acute blood, Leukemia, Promyelocytic, Acute pathology, Molecular Sequence Data, Neoplasm, Residual, Polymerase Chain Reaction, Remission Induction, Biomarkers, Tumor blood, Hematopoietic Stem Cells chemistry, Leukemia, Promyelocytic, Acute diagnosis, Neoplasm Proteins blood, Neoplastic Stem Cells chemistry, Oncogene Proteins, Fusion blood
- Abstract
Detection of minimal residual disease (MRD) by analysis of the PML-RAR alpha fusion transcript using the RT-PCR method is routinely carried out on peripheral blood and bone marrow of patients with APL (AML, FAB:M3). Therapy aims to achieve repeated negative results in these patients thus confirming clinical complete remission. We report a case of APL in second complete remission in which no leukemic cells had been detected in BM and PB for 20 months, and in which PBPC-pheresis was carried out for future transplantation. In two of five pheresis PML-RAR alpha fusion transcripts were detected. This shows that the residual leukemic population may only reach detection level after enrichment by PBPC-pheresis.
- Published
- 1995