1. Exogenous hydrogen sulfide protects against global cerebral ischemia/reperfusion injury via its anti-oxidative, anti-inflammatory and anti-apoptotic effects in rats.
- Author
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Yin J, Tu C, Zhao J, Ou D, Chen G, Liu Y, and Xiao X
- Subjects
- Animals, Biomarkers blood, Blotting, Western, Brain Ischemia pathology, Cerebral Infarction pathology, Enzyme-Linked Immunosorbent Assay, Inflammation metabolism, Inflammation prevention & control, Male, Malondialdehyde metabolism, Oxidative Stress drug effects, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Reperfusion Injury pathology, Signal Transduction drug effects, Stroke, Superoxide Dismutase metabolism, Anti-Inflammatory Agents, Non-Steroidal, Antioxidants, Apoptosis drug effects, Brain Ischemia prevention & control, Hydrogen Sulfide pharmacology, Neuroprotective Agents, Reperfusion Injury prevention & control
- Abstract
The present study was undertaken to study the effects of exogenous hydrogen sulfide (H(2)S) on global cerebral ischemia-reperfusion(I/R) and the underlying mechanisms. After a 24h I/R, administration of NaHS, an exogenous donor for H(2)S, at the dose of 0.2 or 0.4 μmol/kg significantly decreased the apoplexy index, neurological symptom scoring, and brain infarcted area as compared to the I/R group in a dose dependent manner. At the same time, NaHS-treated rats displayed significant reduction of MDA content, and striking increase of SOD activity in the brain tissues compared with I/R group. The up-regulated mRNA levels of p47(phox) and gp91(phox) subunits of NADPH oxidase were also suppressed by NaHS treatment. In this study, the pro-inflammatory markers TNF-α and MCP-1 in I/R group were markedly increased by 24h I/R, which were significantly attenuated by NaHS in a dose-dependent manner. In contrast, the anti-inflammatory factor IL-10 was markedly induced by NaHS administration. In addition, the expression of the anti-apoptotic protein Bcl-2 was significantly decreased in I/R group compared with the sham-operated group. This reduction was significantly blunted in NaHS-treated group. On the contrary, the pro-apoptotic protein Bax content in brain tissues of I/R group was markedly elevated compared with sham-operated animals. And such an induction of Bax content was significantly ameliorated by NaHS. Taken together, our results suggest that hydrogen sulfide has potent protective effect against a severe cerebral injury induced by a global I/R possibly through the inhibition of oxidative stress, inflammation and apoptosis., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2013
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