1. Platelet-derived growth factor (PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-β in murine nucleus tractus solitarius
- Author
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Yoko Ishii, Yuichi Hattori, Masakiyo Sasahara, Yo-ichi Nabeshima, Toshikuni Sasaoka, Toshihiko Fujimori, Akira Haji, Yoshiaki Ohi, and Shigeru Noguchi
- Subjects
medicine.medical_specialty ,Patch-Clamp Techniques ,Becaplermin ,AMPA receptor ,In Vitro Techniques ,Biology ,Neurotransmission ,Inhibitory postsynaptic potential ,Synaptic Transmission ,Receptor, Platelet-Derived Growth Factor beta ,Mice ,chemistry.chemical_compound ,Postsynaptic potential ,Internal medicine ,Reaction Time ,Solitary Nucleus ,medicine ,Animals ,Drug Interactions ,Receptors, AMPA ,Molecular Biology ,6-Cyano-7-nitroquinoxaline-2,3-dione ,Mice, Knockout ,Platelet-Derived Growth Factor ,Analysis of Variance ,musculoskeletal, neural, and ocular physiology ,General Neuroscience ,Solitary nucleus ,Excitatory Postsynaptic Potentials ,Proto-Oncogene Proteins c-sis ,Electric Stimulation ,Phosphoric Monoester Hydrolases ,Cell biology ,Mice, Inbred C57BL ,Endocrinology ,nervous system ,chemistry ,Excitatory postsynaptic potential ,CNQX ,NMDA receptor ,Angiogenesis Inducing Agents ,Neurology (clinical) ,Dizocilpine Maleate ,Excitatory Amino Acid Antagonists ,Developmental Biology - Abstract
Although platelet-derived growth factor (PDGF)-BB activates PDGF receptor-beta (PDGFR-beta) and, in turn, inhibits the glutamate N-methyl-D-aspartate (NMDA) receptor function, whether PDGF-BB modulates the CNS function mediated by another glutamate receptors, alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors, remains poorly understood. Here we now report the inhibitory effect of PDGF-BB on the AMPA receptor function in the nucleus tractus solitarius (NTS) by using slice patch-clamp techniques. Excitatory postsynaptic currents (EPSCs) were evoked by electrical stimulation of the tractus solitarius in mouse NTS second-order neurons. EPSCs were nearly completely eliminated by CNQX but not by MK-801, implying mediation through non-NMDA receptors. PDGF-BB significantly decreased the amplitude of EPSCs without affecting the mean decay time constant. This inhibitory effect was transient and reversible after removing PDGF-BB. Furthermore, PDGF-BB significantly reduced the amplitude of AMPA-induced currents in NTS neurons, which showed that PDGF-BB could suppress the AMPA receptor-mediated excitatory input via the postsynaptic mechanism. The inhibitory effect of PDGF-BB on EPSCs was not observed in mutant mice with conditional deletion of the PDGFR-beta gene in neurons. Together, these studies suggest that the PDGF-B/PDGFR-beta axis inhibits the AMPA receptor-mediated synaptic transmission that comprises the major part of the primary afferent to the NTS second-order neuron. The detected inhibitory action may be involved in the CNS regulation of the respiratory response.
- Published
- 2007