1. Modulation of GFAP mRNA levels following toxic lesions in the basal ganglia of the rat
- Author
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P. Poulat, N. Faucon Biguet, Jacques Mallet, Pierre Rataboul, Alain Privat, Philippe Vernier, Laboratoire de neurobiologie cellulaire et moléculaire (NBCM), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurobiologie du Développement INSERM, and PERIGNON, Alain
- Subjects
Male ,Pathology ,medicine.medical_specialty ,MESH: Hydroxydopamines ,MESH: Rats ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,MESH: Substantia Nigra ,[SDV.NEU.PC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior ,Nigrostriatal pathway ,Substantia nigra ,Striatum ,MESH: Corpus Striatum ,Lesion ,Hydroxydopamines ,Basal ganglia ,Glial Fibrillary Acidic Protein ,medicine ,MESH: Glial Fibrillary Acidic Protein ,Animals ,MESH: Animals ,RNA, Messenger ,Oxidopamine ,Ibotenic Acid ,Oxazoles ,MESH: RNA, Messenger ,Denervation ,[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior ,Glial fibrillary acidic protein ,biology ,General Neuroscience ,MESH: Oxazoles ,[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences ,MESH: Ibotenic Acid ,Rats, Inbred Strains ,GFAP stain ,MESH: Rats, Inbred Strains ,MESH: Male ,Corpus Striatum ,Rats ,MESH: Astrocytes ,MESH: Oxidopamine ,Substantia Nigra ,medicine.anatomical_structure ,nervous system ,Astrocytes ,biology.protein ,medicine.symptom ,[SDV.NEU.SC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences - Abstract
International audience; GFAP mRNA levels were quantified by Northern blot analysis using a human GFAP (glial fibrillary acidic protein) cDNA probe in association with immunocytochemistry. Ten days after a unilateral lesion of substantia nigra with 6-hydroxydopamine (6-OHDA), GFAP mRNA level is increased 1.4-fold in the ipsilateral striatum; thereafter it declined continuously to reach the control level 4 months later. This effect contrasted with the lower and more sustained increase of preproenkephalin (PPE) mRNA, a marker of neuronal target of nigrostriatal pathway. Following ibotenic acid-induced neuronal degeneration of the neostriatum in the rat, we observed a sharp elevation of the GFAP transcripts (4-fold) as soon as 2 days after the lesion both in the striatum and in the substantia nigra. Whereas in the striatum GFAP mRNA level already declined at 5 days postlesion, it remained stable in the substantia nigra. In comparison GFAP immunoreactivity was slightly delayed. No obvious modification was observed in the contralateral side to the lesion whatever the denervation condition studied. Implication of these results on the understanding and the therapeutic approach of glial scarring is discussed.
- Published
- 1989
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