1. Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran
- Author
-
Amir Hossein Zahirnia, Ali Bordbar, Sahar Ebrahimi, Adel Spotin, Somayeh Mohammadi, Seyedeh Maryam Ghafari, Setareh Ahmadvand, Negar Jabbari, Ahmad Reza Esmaeili Rastaghi, and Parviz Parvizi
- Subjects
Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Background: Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis. Methods: All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within. Results: Humans were found to be infected with L. major with high infection frequency and also Leishmania tropica was identified with low occurrence for the first time as non-native species using molecular analyses. The rates of infections was considerable with microscopic observation (n = 65, 73%) out of 89 smears prepared from suspected patients. Molecular analyses showed that the density of L. major was significantly higher (n = 48, 53.93%) than L. tropica (n = 4, 4.49%) (Mann–Whitney U test: p
- Published
- 2018
- Full Text
- View/download PDF