1. Role of a GenoType MTBDRplus line probe assay in early detection of multidrug-resistant tuberculosis at a Brazilian reference center
- Author
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Fernando Bellissimo-Rodrigues, Renata Helena Candido Pocente, Valdes Roberto Bollela, Cinara Silva Feliciano, Lívia Maria Pala Anselmo, and Margarida Maria Passeri do Nascimento
- Subjects
Male ,Medicine (General) ,Genotyping Techniques ,Physiology ,Antitubercular Agents ,Disease ,Biochemistry ,Genotype ,Tuberculosis, Multidrug-Resistant ,General Pharmacology, Toxicology and Pharmaceutics ,Biology (General) ,lcsh:QH301-705.5 ,lcsh:R5-920 ,biology ,Transmission (medicine) ,General Neuroscience ,Isoniazid ,General Medicine ,Female ,Rifampin ,lcsh:Medicine (General) ,Brazil ,medicine.drug ,Adult ,DNA, Bacterial ,medicine.medical_specialty ,Tuberculosis ,QH301-705.5 ,Immunology ,Biophysics ,Ocean Engineering ,Microbial Sensitivity Tests ,Sensitivity and Specificity ,Mycobacterium tuberculosis ,R5-920 ,Multidrug-resistant tuberculosis ,Internal medicine ,medicine ,Humans ,Clinical Investigation ,Line Probe Assay ,Bacteriological Techniques ,business.industry ,Cell Biology ,medicine.disease ,biology.organism_classification ,Multiple drug resistance ,Early Diagnosis ,lcsh:Biology (General) ,Risk factors ,business ,EPIDEMIOLOGIA ,Molecular diagnostic techniques - Abstract
Resistance to Mycobacterium tuberculosis is a reality worldwide, and its diagnosis continues to be difficult and time consuming. To face this challenge, the World Health Organization has recommended the use of rapid molecular tests. We evaluated the routine use (once a week) of a line probe assay (Genotype MTBDRplus) for early diagnosis of resistance and for assessment of the main related risk factors over 2 years. A total of 170 samples were tested: 15 (8.8%) were resistant, and multidrug resistance was detected in 10 (5.9%). The sensitivity profile took 3 weeks (2 weeks for culture and 1 week for rapid testing). Previous treatment for tuberculosis and the persistence of positive acid-fast smears after 4 months of supervised treatment were the major risk factors observed. The use of molecular tests enabled early diagnosis of drug-resistant bacilli and led to appropriate treatment of the disease. This information has the potential to interrupt the transmission chain of resistant M. tuberculosis.
- Published
- 2015