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Bacillus subtilisis a versatile bacterial species able to produce surfactin, a lipopeptide biosurfactant. We carried out the phylogenomic characterization and pangenomic analyses using available B. subtiliscomplete genomes. Also, we report the whole genome of the biosurfactant-producing B. subtilisstrain RI4914 that was isolated from effluent water from an oil exploration field. We applied a hybrid sequencing approach using both long- and short-read sequencing technologies to generate a highly accurate, single-chromosome genome. The pangenomics analysis of 153 complete genomes classified as B. subtilisretrieved from the NCBI shows an open pangenome composed of 28,511 accessory genes, which agrees with the high genetic plasticity of the species. Also, this analysis suggests that surfactin production is a common trait shared by members of this species since the srfAoperon is highly conserved among the B. subtilisstrains found in most of the assemblies available. Finally, increased surfactin production corroborates the higher srfAA gene expression in B. subtilisstrain RI4914.

Here, we report the draft genome sequence and annotation of Nocardia farcinicaTRH1, a petroleum hydrocarbons degrading Actinobacteria isolated from the coastal water of Trindade Island, Brazil.