1. Human papilloma virus DNA: a factor in the pathogenesis of mammary Paget's disease?
- Author
-
Mahmood Manavi, Klaus Czerwenka, Darko Jelincic, Josef Hosmann, Ernst Kubista, Yongxian Lu, and Friedrich Heuss
- Subjects
musculoskeletal diseases ,Cancer Research ,Pathology ,medicine.medical_specialty ,Receptor, ErbB-2 ,Paget's Disease, Mammary ,Mammary gland ,Breast Neoplasms ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Virus ,law.invention ,Pathogenesis ,law ,otorhinolaryngologic diseases ,medicine ,Carcinoma ,Humans ,Papillomaviridae ,Areola ,Polymerase chain reaction ,Oncogene ,Nucleic Acid Hybridization ,medicine.disease ,Immunohistochemistry ,medicine.anatomical_structure ,Oncology ,DNA, Viral ,Carcinogenesis - Abstract
The paraffin sections from 20 nipples with Paget's disease (10 central intraductal and 10 invasive ductal carcinomas) were analyzed for human papilloma virus (HPV) DNA of the low- and intermediate/high-risk groups. Polymerase chain reaction (PCR) and dot (slot) blot hybridization were used for the detection of HPV DNA types 6/11/16/18/31/33/35. In addition, we examined the c-erbB-2 oncogene expression in the specimens to differentiate benign cells in the surface epithelium of the nipple and areola from Paget cells. We found that the oncogene expression of the c-erbB-2 displayed a strong signal in the Paget cells. Using PCR and dot (slot) blot hybridization, we could not detect the HPV DNAs that are specific for the low- and intermediate/high risk-groups in the 20 cases of Paget's disease. Our results showed for the first time that this type of virus did not contribute to the pathogenesis of Paget's disease.
- Published
- 1996