1. Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria
- Author
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I Adeosun, E Adegbite, M Ilori, S. O. Durosaro, C Echefu, A Ajibade, A.O. Adebambo, O.T. Agbebi, and A.B. Ajibike
- Subjects
0301 basic medicine ,Mitochondrial DNA ,Genetic diversity ,food.ingredient ,Phylogenetic tree ,Aquaculture of tilapia ,UPGMA ,Zoology ,Tilapia ,General Medicine ,Biology ,DNA extraction ,03 medical and health sciences ,Diversity index ,030104 developmental biology ,food ,human activities - Abstract
Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software.
- Published
- 2016
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