1. Distinct expression pattern of the full set of secreted phospholipases A2 in human colorectal adenocarcinomas: sPLA2-III as a biomarker candidate
- Author
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Emily J. Greenspan, Gérard Lambeau, Daniel W. Rosenberg, Carine M. Mounier, Wendum D, Fléjou Jf, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Trafic Membranaire et Signalisation Dans les Cellules Epitheliales, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Saint-Antoine (UMRS893), Center for Molecular Medicine, University of Connecticut (UCONN), and Université Nice Sophia Antipolis (1965 - 2019) (UNS)
- Subjects
Male ,Cancer Research ,Pathology ,Colorectal cancer ,Gene Expression ,Polymerase Chain Reaction ,0302 clinical medicine ,MESH: Aged, 80 and over ,Intestinal mucosa ,MESH: Colitis ,MESH: Up-Regulation ,MESH: Group III Phospholipases A2 ,Intestinal Mucosa ,MESH: Aged ,Aged, 80 and over ,0303 health sciences ,MESH: Middle Aged ,secreted phospholipase A2 ,MESH: Phospholipases A2, Secretory ,Group III Phospholipases A2 ,Middle Aged ,Colitis ,Immunohistochemistry ,3. Good health ,Up-Regulation ,qPCR ,Real-time polymerase chain reaction ,Oncology ,colon cancer ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,MESH: Intestinal Mucosa ,Biomarker (medicine) ,Adenocarcinoma ,biomarker ,Female ,Adult ,medicine.medical_specialty ,MESH: Gene Expression ,Colon ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Biology ,03 medical and health sciences ,Survivin ,medicine ,Biomarkers, Tumor ,Humans ,Matrilysin ,MESH: Colon ,Phospholipases A2, Secretory ,Molecular Diagnostics ,030304 developmental biology ,Aged ,MESH: Colonic Neoplasms ,MESH: Humans ,MESH: Adenocarcinoma ,Cancer ,MESH: Adult ,MESH: Immunohistochemistry ,MESH: Polymerase Chain Reaction ,medicine.disease ,MESH: Male ,MESH: Tumor Markers, Biological ,Cancer research ,MESH: Female ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Recent studies suggest that secreted phospholipases A2 (sPLA2s) represent attractive potential tumour biomarkers and therapeutic targets for various cancers. As a first step to address this issue in human colorectal cancer, we examined the expression of the full set of sPLA2s in sporadic adenocarcinomas and normal matched mucosa from 21 patients by quantitative PCR and immunohistochemistry. In normal colon, PLA2G2A and PLA2G12A were expressed at high levels, PLA2G2D, PLA2G5, PLA2G10 and PLA2G12B at moderate levels, and PLA2G1B, PLA2G2F and PLA2G3 at low levels. In adenocarcinomas from left and right colon, the expression of PLA2G3 was increased by up to 40-fold, while that of PLA2G2D and PLA2G5 was decreased by up to 23- and 14-fold. The variations of expression for sPLA2-IID, sPLA2-III and sPLA2-V were confirmed at the protein level. The expression pattern of these sPLA2s appeared to be linked respectively to the overexpression of interleukin-8, defensin alpha6, survivin and matrilysin, and downregulation of SFRP-1 and RLPA-1, all these genes being associated to colon cancer. This original sPLA2 profile observed in adenocarcinomas highlights the potential role of certain sPLA2s in colon cancer and suggests that sPLA2-III might be a good candidate as a novel biomarker for both left and right colon cancers.
- Published
- 2008
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