1. An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase
- Author
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Kohlmann, Alexander, Kipps, Thomas J, Rassenti, Laura Z, Downing, James R, Shurtleff, Sheila A, Mills, Ken I, Gilkes, Amanda F, Hofmann, Wolf-Karsten, Basso, Giuseppe, Dell’Orto, Marta Campo, Foà, Robin, Chiaretti, Sabina, De Vos, John, Rauhut, Sonja, Papenhausen, Peter R, Hernández, Jesus M, Lumbreras, Eva, Yeoh, Allen E, Koay, Evelyn S, Li, Rachel, Liu, Wei-min, Williams, Paul M, Wieczorek, Lothar, and Haferlach, Torsten
- Subjects
Biomedical and Clinical Sciences ,Cardiovascular Medicine and Haematology ,Clinical Research ,Biotechnology ,Rare Diseases ,Cancer ,Hematology ,Adenocarcinoma ,Breast Neoplasms ,Carcinoma ,Europe ,Female ,Gene Expression Profiling ,Humans ,Leukemia ,Liver Neoplasms ,Oligonucleotide Array Sequence Analysis ,Pilot Projects ,RNA ,Reference Standards ,Reproducibility of Results ,Singapore ,United States ,microarray ,gene expression profiling ,leukaemia ,standardization ,diagnostics ,Cardiorespiratory Medicine and Haematology ,Immunology ,Cardiovascular medicine and haematology - Abstract
Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
- Published
- 2008