Wang, C., Liu, Y., Zhang, J., Wang, H.Y., Wu, H.L., Gong, S.M., and He, D.Q.
1. The low-density lipoprotein receptor-related protein 8 (LRP8), a member of the low-density lipoprotein receptor (LDLR) gene family, participates in the supplying of lipid during follicular development. The objective of the study was to identify and characterise theLRP8gene in goose. 2. A 2867 bp fragment that covered the complete coding region (CDS) of goose (Anser cygnoides)LRP8gene was cloned. It encoded a protein of 917 amino acid residues containing a 24-amino acid signal peptide and 5 functional domains. The goose LRP8 showed high nucleic acid and amino acid identities with those in other species. 3. Similarly to duckLRP8gene, two splice variants of LRP8, LRP8-1 (containing 8 ligand-binding repeats) and LRP8-2 (containing 7 ligand-binding repeats), were identified in goose. 4. Semi-quantitative RT-PCR analysis indicates that theLRP8-1transcript is expressed in heart, liver, spleen, lung, kidney, breast muscle, duodenum, hypothalamus, pituitary and ovary, negligible or absent in sebum and oviduct, and theLRP8-2transcript is widely expressed in all examined tissues. 5. A total of 7 SNPs were identified in the coding region of the gooseLRP8gene. [ABSTRACT FROM AUTHOR]