1. [Contribution of Western blotting to the diagnosis of hydatidosis].
- Author
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Makni F, Hachicha L, Mseddi F, Hammami H, Cheikhrouhou F, Sellami H, Sellami A, Mzali R, Boujelbène S, Rebaï R, Beyrouti I, and Ayadi A
- Subjects
- Animals, Antibodies, Helminth blood, Calcinosis diagnosis, Calcinosis parasitology, Central Nervous System Helminthiasis blood, Central Nervous System Helminthiasis diagnosis, Disease Progression, Echinococcosis surgery, Echinococcosis, Hepatic blood, Echinococcosis, Hepatic diagnosis, Echinococcosis, Pulmonary blood, Echinococcosis, Pulmonary diagnosis, Echinococcus immunology, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, Humans, Immunoglobulin G blood, Molecular Weight, Postoperative Period, Sensitivity and Specificity, Blotting, Western, Echinococcosis diagnosis
- Abstract
The aim of this study is to evaluate the contribution of the immunoWesternblot for the diagnosis and the post surgical follow-up of the hydatidosis. 71 sera from patients with hydatidosis confirmed by surgery were studied. All had a negative hydatic serology by screening tests (enzyme-linked immunosorbent assay, hemagglutination, electrosyneresis). 12 patients with sera in pre and post operative were monitored for 2 years. The Echinococcus Western blot IgG permitted to rectify the diagnosis of hydatidosis in 67.6 %. The rate of positivity was 100 % for the multivesicular liver cysts, 60 % for the young cysts and 50 % for the calcified cysts. Western blot permitted to rectify the diagnosis of lung cysts in 62.5 % of cases and in 50 % of cranial-spinal localizations. Analysis of Western Blot evolution in the 12 patients followed in pre and post-surgical revealed the disappearance of the bands 16, 18 and 26-28kDa in 8 month in the 8 patients with complete exeresis. This study proved the value added of Western blot compared to the other traditional techniques for the immunodiagnostic and the post-surgical monitoring of hydatidosis.
- Published
- 2007