1. [Gene amplification and chromosomal rearrangements during acquisition of cellular resistance to the antimetabolite coformycin]
- Author
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Debatisse, Michelle, Toledo, Franck, Buttin, Gérard, Génétique Somatique, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Toledo, Franck
- Subjects
MESH: Gene Rearrangement ,[SDV]Life Sciences [q-bio] ,Drug Resistance ,MESH: Cricetinae ,MESH: Gene Amplification ,MESH: Drug Resistance, Multiple ,MESH: Interphase ,Cricetinae ,MESH: Chromosome Aberrations ,Animals ,MESH: Animals ,MESH: In Situ Hybridization, Fluorescence ,Interphase ,Cells, Cultured ,In Situ Hybridization, Fluorescence ,Chromosome Aberrations ,Gene Rearrangement ,Coformycin ,MESH: Clone Cells ,Gene Amplification ,Fibroblasts ,Drug Resistance, Multiple ,Clone Cells ,[SDV] Life Sciences [q-bio] ,MESH: Karyotyping ,MESH: Fibroblasts ,Karyotyping ,MESH: Drug Resistance ,MESH: Coformycin ,MESH: Sister Chromatid Exchange ,Sister Chromatid Exchange ,MESH: Cells, Cultured - Abstract
International audience; We studied the early stages of gene amplification in a Chinese hamster cell line and we show that two distinct mechanisms can operate at a single locus. Both of them rely on an unequal segregation of gene copies at mitosis. We conclude that cycles of chromatid breakage, followed by fusion of sister chromatids devoid of a telomere that lead to further breaks in mitosis, have a key role in the coupling of gene amplification and genome remodeling. Rearrangements are first limited to a single chromosome but can then potentially spread to any additional chromosome. Occasionally, a sequence containing the selected gene can be looped out, generating a "double minute" and thus initiating an independent process of extrachromosomal amplification.
- Published
- 1994