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Earthquakes trigger other earthquakes and build up clusters in space and time that in turn create a bias in seismic catalogues. Therefore, declustering is considered as a prerequisite in seismic studies, particularly for probabilistic seismic hazard analysis, not only to eliminate the bias but also to decouple mainshocks and triggered events. However, a declustering process is not a straightforward task due to the complex nature of earthquake phenomena. There exist several declustering methods that mostly employ subjective rules to distinguish between background seismicity and offsprings. Eventually, the final declustered catalogues usually deviate significantly according to the employed method. This issue is raising some concerns, such as how to select the most suitable declustering algorithm, or to assess how this selection affects seismic hazard assessment. In consequence, the main goal of this paper is to quantify the sensitivity of seismic hazard assessments to different declustering techniques. Accordingly, the recently compiled Turkish earthquake catalogue was declustered by making use of three declustering algorithms. A total of six declustered catalogues, two catalogues per method, one by implementing the default input parameters, and one by altering the free input parameters of the employed methods, were produced. The clusters of selected earthquakes were studied in terms of the spatial-temporal distribution of earthquake sequences. A sensitivity analysis was conducted through the major steps of seismic hazard assessment for Istanbul metropolitan city. The seismicity of Istanbul and surroundings was modeled on the basis of four areal source zones. Comparative studies showed that, while the selected declustering algorithm did not significantly affect the completeness periods of moderate to large size earthquakes, it considerably altered those of small magnitude events (e.g. Mw 4.3-5.2) and consequently the recurrence parameters of the source zones. Depending on the declustering algorithm and input parameters, the activity rate was observed to vary up to a factor of two. The differences in the declustered catalogues obtained from different declustering approaches resulted in considerable variations in seismic hazard estimations. The hazard maps at return periods of 475 and 2475 years indicated that peak ground acceleration values may vary up to 20% at some locations. Moreover, the differences in 5% damped elastic spectral accelerations at T = 0.2 for the return periods of 475 and 2475 years are about 18 and 12%, respectively, on the southern shores of Istanbul where the highest hazard levels are observed. [ABSTRACT FROM AUTHOR]