1. Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress.
- Author
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Karaduleva EV, Mubarakshina EK, Sharapov MG, Volkova AE, Pimenov OY, Ravin VK, Kokoz YM, and Novoselov VI
- Subjects
- Animals, Heart physiopathology, Heart Rate drug effects, Hydrogen Peroxide adverse effects, Male, Myocardial Contraction drug effects, Perfusion, Peroxidase metabolism, Rats, Rats, Wistar, Superoxide Dismutase metabolism, Antioxidants therapeutic use, Cardiotonic Agents therapeutic use, Myocardial Reperfusion Injury drug therapy, Myocardial Reperfusion Injury prevention & control, Oxidative Stress drug effects, Peroxiredoxins therapeutic use
- Abstract
Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H2O2-induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H2O2-induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.
- Published
- 2016
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