Search

Your search keyword '"S V, Leonov"' showing total 4 results
Start Over Author "S V, Leonov" Journal bulletin of experimental biology and medicine
4 results on '"S V, Leonov"'

3. Phenotypic Characteristics of Dormant Human Non-Small Cell Lung Cancer Cells Surviving Multifraction X-Ray Irradiation

Author

M V, Pustovalova, E I, Yashkina, L, Alhaddad, A N, Osipov, Y, Chen, and S V, Leonov

Subjects
Lung Neoplasms, X-Rays, Carcinoma, Non-Small-Cell Lung, Humans, Tumor Suppressor Protein p53
Abstract

Phenotypic characteristics of human non-small cell lung cancer cells, A549 (p53 wild-type) and H1299 (p53-deficient) as well as their descendants surviving after multifraction X-ray irradiation at a cumulative dose of 60 Gy (sublines A549HR and H1299HR, respectively) were studied before and after additional 2 Gy single dose irradiation. In 24 h after the additional irradiation, we observed a significant increase in the proportion of cells with signs of entosis (by 5 times, p0.05) and SA-β-gal

Published
2022

4. Transcriptomic Analysis of DNA Repair Pathways in Human Non-Small Cell Lung Cancer Cells Surviving Multifraction X-Ray Irradiation

Author

M V, Pustovalova, A A, Guryanova, M I, Sorokin, M V, Suntsova, A A, Buzdin, L, Alhaddad, A N, Osipov, and S V, Leonov

Subjects
Gene Expression Regulation, Neoplastic, Lung Neoplasms, DNA Repair, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, X-Rays, Humans, Tumor Suppressor Protein p53, Transcriptome, Radiation Tolerance
Abstract

Radioresistant sublines of non-small cell lung cancer cells differing in the p53 status were obtained: A549 (p53 wild type) and H1299 (p53 deficient). The exposure to ionizing radiation was carried out using a standard protocol developed on the basis of empirical clinical experience and consisting in exposure in a dose of 2 Gy once a day, 5 days a week up to total dose of 60 Gy. The cells survived after irradiation demonstrated reduced radiosensitivity, as well as changes in differential gene expression in comparison with parental cells. Some differences in the signaling pathways involved in DNA repair were revealed.

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results