1. T lymphocytes from mice immunized with irradiated sporozoites eliminate malaria from hepatocytes.
- Author
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Hoffman SL, Isenbarger D, Long GW, Sedegah M, Szarfman A, Mellouk S, and Ballou WR
- Subjects
- Animals, Antibodies, Cells, Cultured, Interferon-gamma immunology, Liver cytology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Plasmodium berghei radiation effects, Recombinant Proteins, Immunotherapy, Adoptive, Liver parasitology, Plasmodium berghei immunology, T-Lymphocytes, Regulatory immunology
- Abstract
When mice are immunized with radiation-attenuated sporozoites they are solidly protected against sporozoite challenge by an immune response that has been shown to require CD8+ lymphocytes in several strains of mice. The target of this CD8+ T-cell-dependent immunity has not been established. Immune BALB/c mice were shown to develop malaria-specific, CD8+ T-cell-dependent inflammatory infiltrates in their livers after challenge with Plasmodium berghei sporozoites. Spleen cells from immune BALB/c and C57BL/6 mice eliminated hepatocytes infected with the liver stage of P. berghei in vitro. The activity against infected hepatocytes is not inhibited by antibodies to interferon-gamma and is not present in culture supernatants. It is genetically restricted, an indication that malaria antigens on the hepatocyte surface are recognized by immune T-effector cells. Further subunit pre-erythrocytic stage malaria vaccine development will require identification of the antigens recognized by these T cells and a method of immunization that induces such immunity.
- Published
- 1990