1. Association analyses of RANKL/RANK/OPG gene polymorphisms with femoral neck compression strength index variation in Caucasians
- Author
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Xiao-Gang Liu, Jian Zhao, Robert R. Recker, Dong Hai Xiong, Hong-Wen Deng, Liang Wang, Shan-Shan Dong, Yan Guo, Yuan Chen, and Xiang Hong Xu
- Subjects
musculoskeletal diseases ,Adult ,Male ,medicine.medical_specialty ,Bone density ,Compressive Strength ,Endocrinology, Diabetes and Metabolism ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,White People ,Article ,Bone remodeling ,Endocrinology ,Osteoprotegerin ,Bone Density ,Internal medicine ,medicine ,Humans ,Orthopedics and Sports Medicine ,Femoral neck ,Aged ,Bone mineral ,Aged, 80 and over ,Receptor Activator of Nuclear Factor-kappa B ,Femur Neck ,RANK Ligand ,Genetic Variation ,Middle Aged ,United States ,Radiography ,medicine.anatomical_structure ,RANKL ,biology.protein ,Female - Abstract
Femoral neck compression strength index (fCSI), a novel phenotypic parameter that integrates bone density, bone size, and body size, has significant potential to improve hip fracture risk assessment. The genetic factors underlying variations in fCSI, however, remain largely unknown. Given the important roles of the receptor activator of the nuclear factor-kappaB ligand/receptor activator of the nuclear factor-kappaB/osteoprotegerin (RANKL/RANK/OPG) pathway in the regulation of bone remodeling, we tested the associations between RANKL/RANK/OPG polymorphisms and variations in fCSI as well as its components (femoral neck bone mineral density [fBMD], femoral neck width [FNW], and weight). This was accomplished with a sample comprising 1873 subjects from 405 Caucasian nuclear families. Of the 37 total SNPs studied in these three genes, 3 SNPs, namely, rs12585014, rs7988338, and rs2148073, of RANKL were significantly associated with fCSI (P = 0.0007, 0.0007, and 0.0005, respectively) after conservative Bonferroni correction. Moreover, the three SNPs were approximately in complete linkage disequilibrium. Haplotype-based association tests corroborated the single-SNP results since haplotype 1 of block 1 of the RANKL gene achieved an even more significant association with fCSI (P = 0.0003) than any of the individual SNPs. However, we did not detect any significant associations of these genes with fBMD, FNW, or weight. In summary, our findings suggest that the RANKL gene may play an important role in variation in fCSI, independent of fBMD and non-fBMD components.
- Published
- 2008