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Objective: The aim of this study was to analyze the indications of second-the trimester amniocentesis in a tertiary center and evaluate the difference between aseptic techniques before amniocentesis. Material and Methods: The study sample was drawn from the patients who had amniocentesis between 16th and 22th weeks of pregnancy at Trakya University high-risk pregnancy unit between 2015 and 2018. The patients were divided into two groups according to the antiseptic solutions, which used before the operation. Group I comprised of patients in whom 10% povidine- iodine solution was used for aseptic skin preparation. Group II consisted of patients in whom 10% povidine- iodine solution with 70% isopropyl alcohol solution was used. Results: One hundred fifty eight patients were in group I and took 10% povidine- iodine solution was used for aseptic skin preparation before the procedure and 119 (42.9%) patients were in group II and 10% povidine-iodine +2% chlorhexidine gluconate were used for skin preparation. There were no fetal loss in either group. Two patients (0.7%) in group II was admitted to the hospital in the first week after amniocentesis with increased vaginal discharge and slight abdominal pain. Conclusion: Although the lack of evidence for the superiority of any asepsis technique, a combination of aseptic solutions may be an option for the patients with a high risk of fetal loss.