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Your search keyword '"A. Ramírez-Santos"' showing total 8 results
Start Over Author "A. Ramírez-Santos" Journal canadian journal of microbiology
8 results on '"A. Ramírez-Santos"'

3. Identification of σ32-like factors and ftsX-rpoH gene arrangements in enteric bacteria

Author

Jesús Ramírez-Santos and M Carmen Gómez-Eichelmann

Subjects
Immunology, Genetics, General Medicine, Molecular Biology, Applied Microbiology and Biotechnology, Microbiology
Abstract

Western blot analyses using anti-Escherichia coli K-12 σ32 antibodies and Southern blot analyses using rpoH and ftsX DNA probes were performed using different enteric bacteria. Results show that the bacterial strains analysed have σ32-like transcription factors and ftsX and rpoH homologs in a similar map position. Although the presence of σ32-like factors seems to be extended to all Proteobacteria, rpoH and ftsX homologs seem to be present as neighbors in the genome only in the enteric bacteria.Key words: enteric bacteria, heat shock, σ32, ftsX-rpoH.

Published
1998
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4. Identification of σ32-like factors and ftsX-rpoH gene arrangements in enteric bacteria

Author

Gómez-Eichelmann Mc and Jesús Ramírez-Santos

Subjects
Genetics, biology, Hybridization probe, Immunology, General Medicine, biology.organism_classification, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Enterobacteriaceae, Genome, Sigma factor, medicine, bacteria, Proteobacteria, Molecular Biology, Escherichia coli, Bacteria, Southern blot
Abstract

Western blot analyses using anti-Escherichia coli K-12 sigma 32 antibodies and Southern blot analyses using rpoH and ftsX DNA probes were performed using different enteric bacteria. Results show that the bacterial strains analysed have sigma 32-like transcription factors and ftsX and rpoH homologs in a similar map position. Although the presence of sigma 32-like factors seems to be extended to all Proteobacteria, rpoH and ftsX homologs seem to be present as neighbors in the genome only in the enteric bacteria.

Published
1998
Full Text
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5. Expression of the F plasmid ccd toxin-antitoxin system in Escherichia coli cells under nutritional stress

Author

Jesús Ramírez-Santos, Laurence Van Melderen, M. Carmen Gómez-Eichelmann, and Marisela Aguirre-Ramírez

Subjects
Cell division, Immunology, Bacterial Toxins, Lactose, Sigma Factor, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Plasmid, Bacterial Proteins, Transcription (biology), Genetics, medicine, Escherichia coli, Frameshift Mutation, Molecular Biology, urogenital system, Cytotoxins, Escherichia coli Proteins, General Medicine, equipment and supplies, Toxin-antitoxin system, biology.organism_classification, Molecular biology, Culture Media, Lac Operon, Stationary phase, Mutation, Antitoxin, Bacteria
Abstract

The ccd system of the F plasmid encodes CcdB, a protein toxic to DNA-gyrase, and CcdA, its antitoxin. The function attributed to this system is to contribute to plasmid stability by killing bacteria that lose the plasmid during cell division. However, the function of ccd in resting bacteria is not clear. Results presented show that ccd transcription increases as bacteria enter stationary phase and that the amount of the Ccd proteins is higher in bacteria under nutritional stress than in growing bacteria. Moreover, an increase in the frequency of Lac+"adaptive" mutations was observed in stationary-phase bacteria that over-express the Ccd proteins.Key words: ccd system, nutritional stress, adaptive mutation.

Published
2006

6. Identification of sigma 32-like factors and ftsX-rpoH gene arrangements in enteric bacteria

Author

J, Ramírez-Santos and M C, Gómez-Eichelmann

Subjects
DNA, Bacterial, Recombinant Fusion Proteins, Blotting, Western, Cell Cycle Proteins, Sigma Factor, Antibodies, Bacterial, Blotting, Southern, Bacterial Proteins, Enterobacteriaceae, Animals, Electrophoresis, Polyacrylamide Gel, Rabbits, Heat-Shock Proteins, Transcription Factors
Abstract

Western blot analyses using anti-Escherichia coli K-12 sigma 32 antibodies and Southern blot analyses using rpoH and ftsX DNA probes were performed using different enteric bacteria. Results show that the bacterial strains analysed have sigma 32-like transcription factors and ftsX and rpoH homologs in a similar map position. Although the presence of sigma 32-like factors seems to be extended to all Proteobacteria, rpoH and ftsX homologs seem to be present as neighbors in the genome only in the enteric bacteria.

Published
1998

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