1. Internalization ofMycobacterium shottsiiandMycobacterium pseudoshottsiibyAcanthamoeba polyphaga
- Author
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Frederick D. Quinn, Kari Fine-Coulson, David T. Gauthier, Tuhina Gupta, and Russell K. Karls
- Subjects
Bacilli ,food.ingredient ,Immunology ,Acanthamoeba ,Applied Microbiology and Biotechnology ,Microbiology ,Mycobacterium ,Amoeba (genus) ,food ,Bacterial Proteins ,Mycobacterium shottsii ,parasitic diseases ,Genetics ,Trophozoites ,Molecular Biology ,Incubation ,Polyphaga ,Mycobacterium marinum ,Microbial Viability ,biology ,General Medicine ,bacterial infections and mycoses ,biology.organism_classification ,Microscopy, Electron ,Mycobacterium pseudoshottsii - Abstract
Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal growth temperature for these fish isolates is 25 °C. In the present study, amoebae were examined as a potential environmental reservoir for these fish pathogens. Several studies demonstrated that M. avium bacilli replicate within the trophozoite stage and reside in large numbers within the cytosol of the cyst of the free-living amoeba Acanthamoeba polyphaga. Results from the present study showed that M. shottsii, M. pseudoshottsii, and M. marinum bacilli were internalized by A. polyphaga trophozoites within 6 h but that intracellular viability decreased by 2 to 3 logs over 10 days. While an average of 25 M. marinum bacilli were identified by electron microscopy in the cytosol of the cyst
- Published
- 2013
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