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A new disease on greenhouse pepper (Capsicum annuum L.) plants in Culiacan, Mexico with symptoms of chlorosis, wilting and vascular necrosis was observed in 2014. Sixty diseased pepper plants were collected from three different areas of the Culiacan Valley to determine the causal agent of the disease and analyse the potential risk presented by this disease in this region. Fusarium oxysporum was recovered consistently (88% of samples collected) and identified based on cultural, morphological and pathogenicity tests, as well as by PCR amplification and DNA sequencing of the TEF-1α gene. Two pathogenicity tests with 10 monoconidial isolates of F. oxysporum, which were selected randomly from diseased tissues, produced disease symptoms similar to those observed on pepper plants in the greenhouses. Symptoms were different from the typical root rot, wilting and necrosis caused by F. solani and F. oxysporum f. sp. radicis-capsici and instead matched those reported for F. oxysporum f. sp. capsici causing pepper wilt. The results suggest that the causal agent of pepper wilt in the samples analysed was F. oxysporum. This is the first report of pepper wilt caused by this fungus in Mexico, and this disease is a potential threat for pepper production in this region. [ABSTRACT FROM AUTHOR]