1. Silencing of syndecan-binding protein enhances the inhibitory effect of tamoxifen and increases cellular sensitivity to estrogen
- Author
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Xiaojing Guo, Li Fu, Xiaolong Qian, Jun Zhang, Feng Gu, and Fangfang Liu
- Subjects
0301 basic medicine ,Cancer Research ,medicine.drug_class ,Estrogen receptor ,endocrine-therapy resistance ,Syndecan binding ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,breast cancer ,Progesterone receptor ,medicine ,MTT assay ,skin and connective tissue diseases ,Syndecan-binding protein (SDCBP) ,tamoxifen ,Cell growth ,Chemistry ,Cell cycle ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,030104 developmental biology ,Oncology ,Estrogen ,030220 oncology & carcinogenesis ,Cancer research ,Original Article ,Tamoxifen ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Objective: Tamoxifen is used as a complementary treatment for estrogen receptor (ER)-positive breast cancer (BCa), but many patients developed resistance. The aim of this study was to examine the role of syndecan-binding protein (SDCBP) silencing in ER-positive BCa cells.Methods: In MCF-7/T47D cells, the effects of SDCBP silence/overexpression on cell proliferation and estrogenic response were examined. Cell proliferation was examined using the MTT assay and cell cycle regulators were examined by Western blot. Estrogen response was examined from a luciferase activity and evaluation of transcript levels of pS2 and progesterone receptor (PR) upon estrogen administration. Samples of ER-positive BCa were stained with ERα, PR, and SDCBP antibodies, and their expression correlations were analyzed.Results: We found that SDCBP silencing inhibited the proliferation of ER-positive BCa cells and arrested a greater number of cells in the G1 phase of the cell cycle compared to tamoxifen alone, while SDCBP overexpression limited the anti-cancer effects of tamoxifen. SDCBP silencing and overexpression also enhanced and attenuated the estrogenic response, respectively. Expression of SDCBP was negatively correlated with PR, ERα, and the PR/ERα ratio in ER-positive BCa tissue samples.Conclusions: SDCBP may be involved in tamoxifen resistance in ER-positive BCa. Tamoxifen treatment combined with SDCBP silencing may provide a novel treatment for endocrine therapy-resistant BCa.
- Published
- 2018