1. Construction and Validation of a Multi-Institutional Tissue Microarray of Invasive Ductal Carcinoma From Racially and Ethnically Diverse Populations
- Author
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Sylvia Gutierrez, William E. Grizzle, Domenico Coppola, Egiebade Iriabho, Krzysztof Moroz, Chindo Hicks, Gabriel Sica, Stephen O. Sodeke, Edward Seijo, Cathy D. Meade, Jesus Monico, Idhaliz Flores, Ji-Hyun Lee, Jonas S. Almeida, Teresita Muñoz-Antonia, Kate Fisher, Diana S. Lima, Charles Butler, and Margarita Echeverri
- Subjects
0301 basic medicine ,Gerontology ,Adult ,Ethnic group ,Article ,03 medical and health sciences ,0302 clinical medicine ,Ethnicity ,Medicine ,Humans ,Aged ,Aged, 80 and over ,Tissue microarray ,business.industry ,Carcinoma, Ductal, Breast ,Hematology ,General Medicine ,Ethnically diverse ,Middle Aged ,Invasive ductal carcinoma ,Biobank ,Immunohistochemistry ,Health equity ,030104 developmental biology ,Oncology ,Multicenter study ,Tissue Array Analysis ,030220 oncology & carcinogenesis ,Female ,business - Abstract
BackgroundThe scarcity of tissues from racial and ethnic minorities at biobanks poses a scientific constraint to research addressing health disparities in minority populations.MethodsTo address this gap, the Minority Biospecimen/Biobanking Geographic Management Program for region 3 (BMaP-3) established a working infrastructure for a “biobanking” hub in the southeastern United States and Puerto Rico. Herein we describe the steps taken to build this infrastructure, evaluate the feasibility of collecting formalin-fixed, paraffin-embedded tissue blocks and associated data from a single cancer type (breast), and create a web-based database and tissue microarrays (TMAs).ResultsCancer registry data from 6 partner institutions were collected, representing 12,408 entries from 8,279 unique patients with breast cancer (years 2001–2011). Data were harmonized and merged, and deidentified information was made available online. A TMA was constructed from formalin-fixed, paraffin-embedded samples of invasive ductal carcinoma (IDC) representing 427 patients with breast cancer (147 African Americans, 168 Hispanics, and 112 non-Hispanic whites) and was annotated according to biomarker status and race/ethnicity. Biomarker analysis of the TMA was consistent with the literature.ConclusionsContributions from participating institutions have facilitated a robust research tool. TMAs of IDC have now been released for 5 projects at 5 different institutions.
- Published
- 2016