1. A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell
- Author
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Hayato Maeda, Tohru Ohta, Kazuo Miyashita, Michihiro Mutoh, Wataru Murase, Hiroyuki Kojima, Atsuhito Kubota, Mareshige Kojoma, Masaru Terasaki, Mami Takahashi, and Takuya Inoue
- Subjects
Cancer Research ,medicine.medical_treatment ,Pancreatic Intraepithelial Neoplasia ,Apoptosis ,Biochemistry ,Mice ,Pancreatic cancer ,Genetics ,medicine ,Animals ,Humans ,Protein kinase A ,Molecular Biology ,Protein kinase B ,Chemistry ,Kinase ,Cell adhesion molecule ,medicine.disease ,beta Carotene ,Pancreatic Neoplasms ,Disease Models, Animal ,Cytokine ,Cancer research ,Female ,Cytokine receptor ,Carcinoma in Situ ,Research Article - Abstract
Background/aim Fucoxanthinol (FxOH), a predominant metabolite from fucoxanthin (Fx), can exert potential anti-cancer effects in various cancers. However, limited data are available on the effect of FxOH or Fx on pancreatic cancer. The present study investigated the effect of FxOH on a cell line derived from pancreatic cancer tissue developed in Ptf1aCre/+; LSL-k-rasG12D/+ mice. Materials and methods Using flow-cytometric, microarrays, and western blotting analyses, alterations in FxOH-induced apoptosis-related gene expression and protein levels were evaluated in a mice pancreatic cancer cell line, KMPC44. Results FxOH significantly arrested the cells at S phase along with suppression of many gene sets, such as cytokine- cytokine receptor interaction and cell adhesion molecule CAMS. Moreover, attenuated protein levels for cytokine receptors, adhesion, phosphatidylinositol-3 kinase/protein kinase B, and mitogen-activated protein kinase were observed. Conclusion FxOH may prevent pancreatic cancer development in a murine cancer model.
- Published
- 2020