1. Importance of glutamine metabolism in leukemia cells by energy production through TCA cycle and by redox homeostasis
- Author
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Masato Shikami, Hiroshi Miwa, Masakazu Nitta, Mineaki Goto, Motonori Mizutani, Shohei Mizuno, Miyuki Takahashi, Ichiro Hanamura, Norikazu Tsunekawa-Imai, and Kazuto Suganuma
- Subjects
Cancer Research ,Glutamine ,Citric Acid Cycle ,Biology ,chemistry.chemical_compound ,Oxaloacetic acid ,Cell Line, Tumor ,medicine ,Humans ,Glycolysis ,Molecular Targeted Therapy ,Glutaminolysis ,General Medicine ,Glutathione ,medicine.disease ,Cell biology ,Citric acid cycle ,Leukemia ,Leukemia, Myeloid, Acute ,Glucose ,Oncology ,Biochemistry ,chemistry ,Cancer cell ,Energy Metabolism ,Oxidation-Reduction - Abstract
Some cancer cells depend on glutamine despite of pronounced glycolysis. We examined the glutamine metabolism in leukemia cells, and found that HL-60 cells most depended on glutamine in the 4 acute myelogenous leukemia (AML) cell lines examined: growth of HL-60 cells was most suppressed by glutamine deprivation and by inhibition of glutaminolysis, which was rescued by tricarboxylic acid (TCA) cycle intermediate, oxaloacetic acid. Glutamine is also involved in antioxidant defense function by increasing glutathione. Glutamine deprivation suppressed the glutathione content and elevated reactive oxygen species most evidently in HL-60 cells. Glutamine metabolism might be a therapeutic target in some leukemia.
- Published
- 2014