1. Evidence that membrane proteins of rhabdomyosarcoma cell line RD bind human immunodeficiency virus type 1 (HIV-1).
- Author
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Benzair AB, Hirsch I, and Chermann JC
- Subjects
- Animals, Antibodies, Monoclonal, Binding Sites, Blotting, Western, CD4 Antigens immunology, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Polystyrenes, Tumor Cells, Cultured, Viral Proteins metabolism, HIV-1 metabolism, Membrane Proteins metabolism, Neoplasm Proteins metabolism, Rhabdomyosarcoma metabolism
- Abstract
Membrane proteins (MP) obtained from the human mesenchymal rhabdomyosarcoma cell line RD were coated on 96-well polystyrene microplates and tested for their ability to bind human immunodeficiency virus type 1 (HIV-1). The virus bound to MP was detected by solid phase assay. Anti-human CD4 monoclonal antibodies directed against the HIV-1 gp120 binding site of the CD4 receptor did not inhibit viral binding to MP. HIV-1 specific polypeptides were recovered from coated MP to microplates by a modification of the solid phase immunoisolation technique and shown by immunoblotting analysis using a high titer of biotinylated human anti-HIV-1 IgG. Together these findings provide evidence that HIV-1 binding to RD cell surfaces can proceed via a mechanism other than those mediated by the CD4 receptor.
- Published
- 1993
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