1. Lack of prognostic significance of survivin, survivin-deltaEx3, survivin-2B, galectin-3, bag-1, bax-alpha and MRP-1 mRNAs in breast cancer.
- Author
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O'Driscoll L, Linehan R, M Kennedy S, Cronin D, Purcell R, Glynn S, W McDermott E, D Hill A, J O'Higgins N, Parkinson M, and Clynes M
- Subjects
- Adult, Aged, Aged, 80 and over, Antigens, Neoplasm metabolism, Biomarkers, Tumor metabolism, Breast Neoplasms metabolism, Breast Neoplasms pathology, Carcinoma, Ductal genetics, Carcinoma, Ductal metabolism, Carcinoma, Ductal pathology, Carcinoma, Lobular genetics, Carcinoma, Lobular metabolism, Carcinoma, Lobular pathology, Carrier Proteins genetics, Carrier Proteins metabolism, Chemokines, CC genetics, Chemokines, CC metabolism, DNA Primers chemistry, DNA-Binding Proteins, Female, Galectin 3 genetics, Galectin 3 metabolism, Gene Expression Regulation, Neoplastic, Humans, Inhibitor of Apoptosis Proteins, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Middle Aged, Neoplasm Invasiveness genetics, Neoplasm Invasiveness pathology, Neoplasm Proteins, Prognosis, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins metabolism, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, RNA, Neoplasm metabolism, Reverse Transcriptase Polymerase Chain Reaction, Survival Rate, Survivin, Transcription Factors, bcl-2-Associated X Protein, Antigens, Neoplasm genetics, Biomarkers, Tumor genetics, Breast Neoplasms genetics, RNA, Messenger metabolism
- Abstract
The expression of transcripts for anti-apoptotic (survivin, survivin-deltaEx3, survivin-2B, galectin-3, bag-1 and bcl-2) and pro-apoptotic (bax-alpha) genes, and for multiple drug resistance related protein-1 (MRP-1) gene were investigated, using RT-PCR, in 106 breast tumour biopsies. Normal breast tissue was also analysed for comparative purposes. Overall, survivin, survivin-deltaEx3, survivin-2B, bcl-2, bag-1, galectin-3, bax-alpha and MRP-1 mRNAs were detected in 68, 54.7, 9.4, 78.4, 80.9, 98.9, 97.8 and 72.8%, respectively, of tumour specimens. Uniquely among the mRNAs analysed, the expression of bcl-2 correlated significantly with disease outcome, with bcl-2 expression indicative of favourable outcome in terms of both relapse-free survival and overall survival. This suggests that bcl-2 mRNA expression may be a key prognostic marker for breast cancer and that routine analysis of expression of this transcript should be considered. The results from this study suggest, however, that the expression of survivin, survivin-deltaEx3, survivin-2B, bag-1, galectin-3, bax-alpha and MRP-1 mRNAs cannot be considered as prognostic indicators of disease outcome for patients with breast cancer.
- Published
- 2003
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