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Your search keyword '"Fei-Fei Nie"' showing total 2 results
Start Over Author "Fei-Fei Nie" Journal cancer letters
2 results on '"Fei-Fei Nie"'

1. MAC related mitochondrial pathway in oroxylin A induces apoptosis in human hepatocellular carcinoma HepG2 cells

Author

Na Lu, Fei-Fei Nie, Jun Wang, Jing-Jing Rong, Wei Liu, Qidong You, Qinglong Guo, Qinsheng Dai, Rong Mu, Yong Yang, Rong Hu, Qi Qi, and Xiaotang Wang

Subjects
Cancer Research, Carcinoma, Hepatocellular, Recombinant Fusion Proteins, Apoptosis, Mitochondria, Liver, Mitochondrion, Mitochondrial Membrane Transport Proteins, Ion Channels, Mitochondrial membrane transport protein, chemistry.chemical_compound, Bcl-2-associated X protein, Cell Line, Tumor, Humans, RNA, Small Interfering, bcl-2-Associated X Protein, Flavonoids, Membrane Potential, Mitochondrial, biology, Mitochondrial Permeability Transition Pore, Liver Neoplasms, biology.organism_classification, Antineoplastic Agents, Phytogenic, Neoplasm Proteins, Cell biology, Protein Transport, Proto-Oncogene Proteins c-bcl-2, Oncology, Mitochondrial permeability transition pore, Biochemistry, chemistry, Cell culture, Mitochondrial Membranes, Cyclosporine, biology.protein, Scutellaria baicalensis, Oroxylin A, RNA Interference, Dimerization
Abstract

Oroxylin A is a flavonoid isolated from the root of Scutellaria baicalensis Georgi. Our previous work demonstrated that the anti-tumor activity of oroxylin A was mainly attributed to its apoptosis inducing effect in cells. The present study explores the exact molecular mechanism of oroxylin A-induced apoptosis in tumor cells. We showed that oroxylin A-induced apoptosis in HepG2 cells was achieved through mitochondrial pathway. We also investigated which mitochondrial channels, PTP or MAC or both, were involved in the permeabilization of the mitochondrial outer membrane after treatment with oroxylin A. The results showed that oroxylin A-induced apoptosis in a PTP-independent manner; therefore, we focused our attention on MAC. As Bax is an essential constituent of MAC in certain systems, we examined the activation, subcellular location, oligomeric structure of Bax in HepG2 cells treated with oroxylin A. Moreover, our results showed that overexpression of Bcl-2 inhibited oroxylin A-induced apoptosis. In summary, we have demonstrated that opening of MAC, but not PTP, played a key role in oroxylin A-induced activation of mitochondrial apoptotic pathway in HepG2 cells.

Published
2009

2. Differential apoptotic induction of gambogic acid, a novel anticancer natural product, on hepatoma cells and normal hepatocytes

Author

Yong Yang, Xiaotang Wang, Lan Yang, Fei-Fei Nie, Hong-Yan Gu, Qidong You, Li Zhao, and Qinglong Guo

Subjects
Male, Cancer Research, Carcinoma, Hepatocellular, Xanthones, Blotting, Western, Mice, Nude, Caspase 3, Apoptosis, Rats, Sprague-Dawley, chemistry.chemical_compound, Mice, Bcl-2-associated X protein, Cell Line, Tumor, Animals, Humans, Garcinia, Cell Shape, Cells, Cultured, Cell Proliferation, bcl-2-Associated X Protein, Mice, Inbred BALB C, biology, Dose-Response Relationship, Drug, Cell growth, Liver Neoplasms, biology.organism_classification, Antineoplastic Agents, Phytogenic, Xenograft Model Antitumor Assays, Cell biology, Rats, Oncology, Biochemistry, chemistry, Proto-Oncogene Proteins c-bcl-2, Garcinia hanburyi, biology.protein, Hepatocytes, Gambogic acid, Female, Growth inhibition
Abstract

Gambogic acid (GA) is the major active ingredient of gamboge, a brownish resin exuded from Garcinia hanburryi tree in Southeast Asia. In this study, we compared the different apoptotic induction of GA on human normal embryonic hepatic L02 cells and human hepatoma SMMC-7721 cells by detecting growth inhibition, observing morphological changes, and the expressions of the relative apoptotic proteins (Bax, Bcl-2 and caspase-3). The results indicated that GA could selectively induce apoptosis of SMMC-7721 cells, while had relatively less effect on L02 cells. To illustrate the distinct selective antitumor mechanism of GA, we further study its distribution in cultured cells and in tumor-bearing mice. The results indicated that SMMC-7721 cells have higher GA binding activity than L02 cells. The retention time of GA in grafted tumor was longer than in liver, renal and other organs. Collectively, the selective anticancer activity of GA could be due to its significant apoptotic inducing effects as well as its higher distribution and longer retention time in tumor cells compared to the normal cells. So GA might be a kind of highly effective anticancer drug candidate with low toxicity to normal tissue.

Published
2007

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